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Bacterial DNA synthesized under phage control in a DNA-defective Salmonella-mutant and packaged into a special fraction of transducing particles of phage P22

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Summary

Lysates of P22 contain a small fraction of transducing particles with bacterial DNA replicated semiconservatively after the time of infection. It was demonstrated that the presence and relative amount of this class of transducing particles was unchanged, if infection of Salmonella occured under a condition nonpermissive for bacterial DNA replication. Analysis of particles with DNA fragments derived from different regions of the Salmonella chromosome indicated that the replication of the bacterial DNA carried by these transducing particles was not initiated specifically at the normal origin for bacterial chromosome replication.

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Abbreviations

i.p.:

transducing particles

moi:

multiplicity of infection

i.p.:

intectious particles

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Authors and Affiliations

  1. Genetisches Institut der Universität München, Maria-Ward-Str. 1a, D-8000, München 19, Federal Republic of Germany

    Horst Backhaus & Horst Schmieger

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  1. Horst Backhaus
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  2. Horst Schmieger
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Communicated by T. Yura

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Backhaus, H., Schmieger, H. Bacterial DNA synthesized under phage control in a DNA-defective Salmonella-mutant and packaged into a special fraction of transducing particles of phage P22. Molec. Gen. Genet. 171, 301–306 (1979). https://doi.org/10.1007/BF00267585

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  • DOI: https://doi.org/10.1007/BF00267585

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