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Pyramiding of Ryd2 and Ryd3 conferring tolerance to a German isolate of Barley yellow dwarf virus-PAV (BYDV-PAV-ASL-1) leads to quantitative resistance against this isolate

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Abstract

Barley yellow dwarf virus (BYDV) is an economically important pathogen of barley, which may become even more important due to global warming. In barley, several loci conferring tolerance to BYDV-PAV-ASL-1 are known, e.g. Ryd2, Ryd3 and a quantitative trait locus (QTL) on chromosome 2H. The aim of the present study was to get information whether the level of tolerance against this isolate of BYDV in barley can be improved by combining these loci. Therefore, a winter and a spring barley population of doubled haploid (DH) lines were genotyped by molecular markers for the presence of the susceptibility or the resistance encoding allele at respective loci (Ryd2, Ryd3, QTL on chromosome 2H) and were tested for their level of BYDV-tolerance after inoculation with viruliferous (BYDV-PAV-ASL-1) aphids in field trials. In DH-lines carrying the combination Ryd2 and Ryd3, a significant reduction of the virus titre was detected as compared to lines carrying only one of these genes. Furthermore, spring barley DH-lines with this allele combination also showed a significantly higher relative grain yield as compared to lines carrying only Ryd2 or Ryd3. The QTL on chromosome 2H had only a small effect on the level of tolerance in those lines carrying only Ryd2, or Ryd3 or a combination of both, but the effect in comparison to lines carrying no tolerance allele was significant. Overall, these results show that the combination of Ryd2 and Ryd3 leads to quantitative resistance against BYDV-PAV instead of tolerance.

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Acknowledgment

The authors thank the Federal Ministry for Nutrition, Agriculture and Consumer Protection (BMELV) and the Gemeinschaft zur Förderung der privaten deutschen Pflanzenzüchtung e.V (GFP) for financial support (PGI-06.01-28-1-41.002-06). For carrying out field trials we thank Dr. Claus Einfeldt from Saatzucht Dr. J. Ackermann & Co, Irlbach, Dr. Viktor Korzun from KWS-Lochow GmbH, Dr. Eberhard Laubach from Nordsaat Saatzuchtgesellschaft mbH and Dr. Jens Weyen from Saaten-Union Biotec GmbH for producing the DH-lines.

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Authors and Affiliations

  1. Julius Kuehn-Institute (JKI), Federal Research Institute for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany

    Christine Riedel, Antje Habekuß, Edgar Schliephake & Frank Ordon

  2. Department of Plant Breeding, Graduate School of Experimental Plant Sciences, Wageningen University, P.O. Box 386, 6700, Wageningen, The Netherlands

    Rients Niks

  3. Faculty of Agricultural and Environmental Sciences, Institute for Land Use, Agrobiotechnology and Applied Research in Bio- and Gene-Technology, University of Rostock, Justus-von-Liebig-Weg 8, 18059, Rostock, Germany

    Inge Broer

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  1. Christine Riedel
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  2. Antje Habekuß
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  3. Edgar Schliephake
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  4. Rients Niks
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  5. Inge Broer
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  6. Frank Ordon
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Correspondence to Frank Ordon.

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Communicated by P. Hayes.

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Riedel, C., Habekuß, A., Schliephake, E. et al. Pyramiding of Ryd2 and Ryd3 conferring tolerance to a German isolate of Barley yellow dwarf virus-PAV (BYDV-PAV-ASL-1) leads to quantitative resistance against this isolate. Theor Appl Genet 123, 69–76 (2011). https://doi.org/10.1007/s00122-011-1567-y

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