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Large scale biomimetic membrane arrays

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Abstract

To establish planar biomimetic membranes across large scale partition aperture arrays, we created a disposable single-use horizontal chamber design that supports combined optical–electrical measurements. Functional lipid bilayers could easily and efficiently be established across CO2 laser micro-structured 8 × 8 aperture partition arrays with average aperture diameters of 301 ± 5 μm. We addressed the electro-physical properties of the lipid bilayers established across the micro-structured scaffold arrays by controllable reconstitution of biotechnological and physiological relevant membrane peptides and proteins. Next, we tested the scalability of the biomimetic membrane design by establishing lipid bilayers in rectangular 24 × 24 and hexagonal 24 × 27 aperture arrays, respectively. The results presented show that the design is suitable for further developments of sensitive biosensor assays, and furthermore demonstrate that the design can conveniently be scaled up to support planar lipid bilayers in large square-centimeter partition arrays.

Fluorescent image of a large 24 × 24 rectangular bilayer array

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Abbreviations

α-HL:

α-Hemolysin

BLM:

Black lipid membrane

DPhPC:

1,2-Diphytanoyl-sn-Glycero-3-Phosphocholine

ETFE:

Ethylene tetrafluoroethylene

FomA:

Fusobacterium nucleatum outer membrane protein A

gA:

Gramicidin A

LDAO:

N-lauryl-N,N-dimethylammonium-N-oxide

NBD-PC:

1-Oleoyl-2-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-sn-glycero-3-phosphocholine

TEA:

Tetraethylammonium

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Acknowledgments

We thank Dr. Jörg H. Kleinschmidt (Universität Konstanz, Germany) for the delivery and guidance with the handling and incorporation of FomA porins. The work was supported through MEMBAQ, a Specific Targeted Research Project (STREP), by the European Commission under the Sixth Framework Program (NMP4-CT-2006-033234), by The Danish National Advanced Technology Foundation (023-2007-1), and The Danish National Research Foundation.

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Authors and Affiliations

  1. DTU Nanotech, Technical University of Denmark, 2800, Lyngby, Denmark

    Jesper S. Hansen, Jörg Vogel, Oliver Geschke & Jenny Emneús

  2. Aquaporin A/S, Scion DTU, Diplomvej 377, 2800, Lyngby, Denmark

    Jesper S. Hansen, Mark Perry, Jörg Vogel, Jesper S. Groth, Thomas Vissing, Marianne S. Larsen & Claus H. Nielsen

  3. DTU Physics, Quantum Protein Center, Technical University of Denmark, 2800, Lyngby, Denmark

    Henrik Bohr & Claus H. Nielsen

Authors
  1. Jesper S. Hansen
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  2. Mark Perry
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  3. Jörg Vogel
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  5. Thomas Vissing
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  7. Oliver Geschke
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  9. Henrik Bohr
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  10. Claus H. Nielsen
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Correspondence to Claus H. Nielsen.

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Hansen, J.S., Perry, M., Vogel, J. et al. Large scale biomimetic membrane arrays. Anal Bioanal Chem 395, 719–727 (2009). https://doi.org/10.1007/s00216-009-3010-7

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  • DOI: https://doi.org/10.1007/s00216-009-3010-7

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