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Determination of stilbene derivatives in Burgundy red wines by ultra-high-pressure liquid chromatography

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Abstract

The polyphenols, for example stilbenes and flavonoids, are an important family of compounds present in grapes and wines. Several studies have shown that stilbenes are antioxidants and cancer-preventing agents. For the first time, eight natural stilbenes (trans-resveratrol, trans-piceid, cis-piceid, trans-astringin, trans-piceatannol, (+)-trans-ε-viniferin, pallidol, and hopeaphenol), isolated and purified from Vitis vinifera, were simultaneously analysed by ultra-high-pressure liquid chromatography coupled with photodiode-array detection. Separation of the stilbenes by UHPLC was optimized with the assistance of “Quality-by-Design” commercial software. Four different reversed-phase columns packed with 1.5–1.7-μm particles were tested and compared for their retention behaviour and separation efficiency. On the basis of the performance characteristics determined, the VisionHT C18 HL column was selected for the stilbenes studied, because resolution of the critical pair was 1.5 with a peak width of 2–4 s. The optimized method resulted in highly repeatable retention times (RSD 0.03–0.07%), peak areas (RSD 3–6%), and linear ranges were between 0.005 and 50 mg L−1 for most of the compounds. All stilbenes, except trans-astringin, trans-piceatannol, and pallidol were identified and quantified in Burgundy red wines at different concentrations after direct injection of the wines.

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Acknowledgements

We thank the Maison Louis Jadot from Beaune for providing wine samples. This work was financially supported by the Région Bourgogne, the Bureau Interprofessionnel des Vins de Bourgogne, and the Centre de Coopération Universitaire Franco-Bavarois.

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Correspondence to Philippe Schmitt-Kopplin.

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Published in the special issue Wine Analysis with Guest Editor Isabelle Pianet

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Boutegrabet, L., Fekete, A., Hertkorn, N. et al. Determination of stilbene derivatives in Burgundy red wines by ultra-high-pressure liquid chromatography. Anal Bioanal Chem 401, 1513–1521 (2011). https://doi.org/10.1007/s00216-011-4879-5

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  • DOI: https://doi.org/10.1007/s00216-011-4879-5

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