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Interactions Between Hyphosphere-Associated Bacteria and the Fungus Cladosporium herbarum on Aquatic Leaf Litter

  • Fungal Microbiology
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Abstract

We investigated microbial interactions of aquatic bacteria associated with hyphae (the hyphosphere) of freshwater fungi on leaf litter. Bacteria were isolated directly from the hyphae of fungi from sedimented leaves of a small stream in the National Park “Lower Oder,” Germany. To investigate interactions, bacteria and fungi were pairwise co-cultivated on leaf-extract medium and in microcosms loaded with leaves. The performance of fungi and bacteria was monitored by measuring growth, enzyme production, and respiration of mono- and co-cultures. Growth inhibition of the fungus Cladosporium herbarum by Ralstonia pickettii was detected on leaf extract agar plates. In microcosms, the presence of Chryseobacterium sp. lowered the exocellulase, endocellulase, and cellobiase activity of the fungus. Additionally, the conversion of leaf material into microbial biomass was retarded in co-cultures. The respiration of the fungus was uninfluenced by the presence of the bacterium.

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Acknowledgements

We are very grateful to Maike Mai (TU Berlin Department of Waste Management and Environmental Research) who attended the respiration rate measurements. We are also very thankful for the aid of Sabine Rautenberg (TU Berlin Department of Soil Science) with the chromatography analysis. We thank Joseph Bishop of the University of Missouri-Rolla, USA for revising the English language of the manuscript.

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Authors and Affiliations

  1. Department of Environmental Microbiology, Technische Universität Berlin, Franklinstraße 29, 10587, Berlin, Germany

    Christiane Baschien & Ulrich Szewzyk

  2. Gesellschaft zur Förderung von Medizin-, Bio- und Umwelttechnologien e.V., Funktionelle Schichten (GMBU e.V.), Bautzner Landstraße 45, 01454, Großerkmannsdorf, Germany

    Georg Rode

  3. Berliner Wasserbetriebe, Motardstrasse 35, 13629, Berlin, Germany

    Uta Böckelmann

  4. Institut für Bioverfahrenstechnik, Universität Stuttgart, Allmandring 31, 70569, Stuttgart, Germany

    Peter Götz

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  1. Christiane Baschien
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  2. Georg Rode
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  3. Uta Böckelmann
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Correspondence to Christiane Baschien.

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Baschien, C., Rode, G., Böckelmann, U. et al. Interactions Between Hyphosphere-Associated Bacteria and the Fungus Cladosporium herbarum on Aquatic Leaf Litter. Microb Ecol 58, 642–650 (2009). https://doi.org/10.1007/s00248-009-9528-6

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