Abstract
Sulfadiazine (SDZ) is an antibiotic frequently used in agricultural husbandry. Via manuring of excrements of medicated animals, the drug reaches the soil and might impair important biochemical transformation processes performed by microbes, e.g., the nitrogen turnover. We studied the effect of pig manure and SDZ-spiked pig manure on denitrifying bacteria by quantifying nirK and nirS nitrite reductase genes in two arable soils. Addition of manure entailed mainly an increase of nirK-harboring denitrifiers in both soils, whereas in the SDZ-amended treatments, primarily the nirS denitrifiers increased in abundance after the bioavailable SDZ had declined. However, the community composition of nirS nitrite reducers investigated by denaturing gradient gel electrophoresis did not change despite the observed alterations in abundance.
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References
Alcock RE, Sweetman A, Jones KC (1999) Assessment of organic contaminant fate in waste water treatment plants: I. Selected compounds and physicochemical properties. Chemosphere 38:2247–2262
Braker G, Fesefeldt A, Witzel K-P (1998) Development of PCR primer systems for amplification of nitrite reductase genes (nirK and nirS) to detect denitrifying bacteria in environmental samples. Appl Environ Microbiol 64:3769–3775
Brown GM (1962) The Biosynthesis of folic acid. II. Inhibition by sulfonamides. J Biol Chem 237:536–540
Burkhardt M, Stamm C, Waul C, Singer H, Muller S (2005) Surface runoff and transport of sulfonamide antibiotics and tracers on manured grassland. J Environ Qual 34:1363–1371
Dambreville C, Hallet S, Nguyen C, Morvan T, Germon J-C, Philippot L (2006) Structure and activity of the denitrifying community in a maize-cropped field fertilized with composted pig manure or ammonium nitrate. FEMS Microbiol Ecol 56:119–131
Dendooven L, Bonhomme E, Merckx R, Vlassak K (1998) N dynamics and sources of N2O production following pig slurry application to a loamy soil. Biol Fertil Soils 26:224–228
Elmund GK, Morrison SM, Grant DW, Nevins MP (1971) Role of excreted chlortetracycline in modifying the decomposition process in feedlot waste. Bull Environ Contam Toxicol V6:129–132
Griffiths RI, Whiteley AS, O'Donnell AG, Bailey MJ (2000) Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition. Appl Environ Microbiol 66:5488–5491
Halling-Sørensen B (2001) Inhibition of aerobic growth and nitrification of bacteria in sewage sludge by antibacterial agents. Arch Environ Contam Toxicol V40:451–460
Hammesfahr U, Heuer H, Manzke B, Smalla K, Thiele-Bruhn S (2008) Impact of the antibiotic sulfadiazine and pig manure on the microbial community structure in agricultural soils. Soil Biol Biochem 40:1583–1591
Hamscher G, Sczesny S, Höper H, Nau H (2002) Determination of persistent tetracycline residues in soil fertilized with liquid manure by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry. Anal Chem 74:1509–1518
Heuer H, Smalla K (2007) Manure and sulfadiazine synergistically increased bacterial antibiotic resistance in soil over at least two months. Environ Microbiol 9:657–666
Kotzerke A, Sharma S, Schauss K, Heuer H, Thiele-Bruhn S, Smalla K, Wilke B-M, Schloter M (2008) Alterations in soil microbial activity and N-transformation processes due to sulfadiazine loads in pig-manure. Environ Pollut 153:315–322
Lowrance R, Williams RG, Johnson JC Jr, Newton GL (1998) Denitrification from soils of a year-round forage production system fertilized with liquid dairy manure. J Environ Qual 27:1504–1511
Michotey V, Méjean V, Bonin P (2000) Comparison of methods for quantification of cytochrome cd 1-denitrifying bacteria in environmental marine samples. Appl Environ Microbiol 66:1564–1571
Paul JW, Beauchamp EG (1989) Effect of carbon constituents in manure on denitrification in soil. Can J Soil Sci 69:49–61
Philippot L, Hallin S, Schloter M (2007) Ecology of denitrifying prokaryotes in agricultural soil. In: Sparks DL (ed) Adv Agron, vol. 96. Academic Press, Amsterdam, pp 135–191
Schauss K, Focks A, Leininger S, Kotzerke A, Heuer H, Thiele-Bruhn S, Sharma S, Wilke B-M, Matthies M, Smalla K, Munch JC, Amelung W, Kaupenjohann M, Schloter M, Schleper C (2009) Dynamics and functional relevance of ammonia-oxidizing archaea in two agricultural soils. Environ Microbiol 11:446–456
Sharma S, Aneja MK, Mayer J, Munch JC, Schloter M (2005) Diversity of transcripts of nitrite reductase genes (nirK and nirS) in rhizospheres of grain legumes. Appl Environ Microbiol 71:2001–2007
Thompson RB (1989) Denitrification in slurry-treated soil: occurrence at low temperatures, relationship with soil nitrate and reduction by nitrification inhibitors. Soil Biol Biochem 21:875–882
Throbäck IN, Enwall K, Jarvis Å, Hallin S (2004) Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitifying bacteria with DGGE. FEMS Microbiol Ecol 49:401–417
Winckler C, Grafe A (2001) Use of veterinary drugs in intensive animal production. J Soils Sediments 1:66–70
Acknowledgements
The authors gratefully acknowledge the funding by the German Research Foundation DFG as a part of the research group FOR566 “Veterinary medicines in soils.”
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Kristina Kleineidam and Shilpi Sharma contributed equally.
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Kleineidam, K., Sharma, S., Kotzerke, A. et al. Effect of Sulfadiazine on Abundance and Diversity of Denitrifying Bacteria by Determining nirK and nirS Genes in Two Arable Soils. Microb Ecol 60, 703–707 (2010). https://doi.org/10.1007/s00248-010-9691-9
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DOI: https://doi.org/10.1007/s00248-010-9691-9