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Fungal microsomes in a biotransformation perspective: protein nature of membrane-associated reactions

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Abstract

Microsomal fraction of fungal cells grabs the attention of many researchers for it contains enzymes that play a role in biotechnologically relevant processes. Microsomal enzymes, namely, CYP450s, were shown to metabolize a wide range of xenobiotic compounds, including PAHs, PCBs, dioxins, and endocrine disruptors, and take part in other fungal biotransformation reactions. However, little is known about the nature and regulation of these membrane-associated reactions. Advanced proteomic and post-genomic techniques make it possible to identify larger numbers of microsomal proteins and thus add to a deeper study of fungal intracellular processes. In this work, proteins that were identified through a shotgun proteomic approach in fungal microsomes under various culture conditions are reviewed. However, further research is still needed to fully understand the role of microsomes in fungal biodegradation and biotransformation reactions.

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Acknowledgments

This work was supported by the projects DAAD A/13/07824, TE01020218 of the Czech Technology Agency and the Institutional Research Concept RVO: 61388971.

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Authors and Affiliations

  1. Laboratory of Environmental Biotechnology, Institute of Microbiology ASCR, v.v.i., Videnska 1083, 14220, Prague, Czech Republic

    Kateřina Svobodová & Hana Mikesková

  2. Institute of Chemical Technology Prague, Faculty of Food and Biochemical Technology, Technická 5, 160 28, Prague 6, Czech Republic

    Hana Mikesková

  3. Laboratory of Cell Signalization, Institute of Microbiology ASCR, v.v.i., Videnska 1083, 14220, Prague, Czech Republic

    Denisa Petráčková

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  3. Denisa Petráčková
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Correspondence to Kateřina Svobodová.

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Svobodová, K., Mikesková, H. & Petráčková, D. Fungal microsomes in a biotransformation perspective: protein nature of membrane-associated reactions. Appl Microbiol Biotechnol 97, 10263–10273 (2013). https://doi.org/10.1007/s00253-013-5347-2

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  • DOI: https://doi.org/10.1007/s00253-013-5347-2

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