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Low expression of chemokine receptor CCR5 in human colorectal cancer correlates with lymphatic dissemination and reduced CD8+ T-cell infiltration

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Background

Chemokines and their receptors have been proposed to distinctly contribute to tumor growth, dissemination, and local immune escape. The aim of this study was to evaluate the relevance of the chemokine receptor CCR5 expression for the progression of human colorectal cancer.

Methods

CCR5 expression was assessed by RT-PCR analysis in 103 colorectal cancer patients. Intensity of CCR5 expression was correlated with both tumor and patient characteristics. Infiltration of tumor margins with CD8+ T cells in the context of CCR5 expression was analyzed by immunohistochemistry in additional 18 colorectal cancer specimens.

Results

Human colorectal cancer revealed variable intensities of CCR5 expression ranging from absent (48/103: 47%), weak (30/103: 29%), intermediate (13/103: 13%), to strong (12/103: 12%). Absent or weak CCR5 expression was significantly associated with advanced UICC stages (P = 0.02) and lymphatic metastasis (P = 0.05). In addition, CCR5 expression positively correlated with CD8+ T-cell infiltration in tumor margins (P = 0.001).

Conclusion

In summary, intermediate and strong CCR5 expression was significantly associated with nonmetastatic colorectal cancer and increased CD8+ T-cell infiltration.

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Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. First Department of Internal Medicine, Johannes Gutenberg University of Mainz, Langenbeck Strasse 1, 55101, Mainz, Germany

    Tim Zimmermann, Markus Moehler, Peter R. Galle & Carl C. Schimanski

  2. Department of General and Abdominal Surgery, University of Mainz, Mainz, Germany

    Ines Gockel, George G. Sgourakis & Hauke Lang

  3. The Interdisciplinary Translational Oncological Laboratorium (ITOL), Johannes Gutenberg University of Mainz, Mainz, Germany

    Markus Moehler, Ines Gockel, George G. Sgourakis & Carl C. Schimanski

  4. AG Toxicology and Chemotherapy, German Cancer Research Institute, Heidelberg, Germany

    Martin R. Berger

  5. Institute of Pathology, University of Mainz, Mainz, Germany

    Stefan Biesterfeld

  6. Department of Gastroenterology, German Diagnostic Clinic, Wiesbaden, Germany

    Michaela Müller

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  1. Tim Zimmermann
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  2. Markus Moehler
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  10. Carl C. Schimanski
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Correspondence to Carl C. Schimanski.

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Zimmermann, T., Moehler, M., Gockel, I. et al. Low expression of chemokine receptor CCR5 in human colorectal cancer correlates with lymphatic dissemination and reduced CD8+ T-cell infiltration. Int J Colorectal Dis 25, 417–424 (2010). https://doi.org/10.1007/s00384-009-0868-y

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  • DOI: https://doi.org/10.1007/s00384-009-0868-y

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