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Comparative analysis of oncofetal fibronectin and tenascin-C expression in right atrial auricular and left ventricular human cardiac tissue from patients with coronary artery disease and aortic valve stenosis

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Abstract

Aortic valve stenosis (AVS) and coronary artery disease (CAD) are accompanied by changes in the cardiac extra cellular matrix (cECM) including the re-expression of oncofetal fibronectin (Fn) and tenascin-C (Tn-C) variants. Human antibodies against these variants are usable for targeted therapy. Aim of the study was the comparative analysis of cECM remodelling in tissue samples from right atrial auricle (RAA) and left ventricular septum (LVS). RAA and LVS specimens from 30 patients (17 × AVS; 13 × AVS+CAD) were analysed with respect to histological changes and ECM remodelling using PCR based ECM gene expression profiling. Re-expression of ED-A+ Fn and A1+ Tn-C was investigated on the mRNA and on the protein level. For immunofluorescence, human recombinant small immunoprotein (SIP) format antibodies were used. There was a positive correlation of the grade of histological changes in RAA and corresponding LVS samples (r = 0.695). ECM gene expression levels were higher in LVS compared to RAA. For 24 genes, a corresponding relevant (>2.5-fold) up- or down-regulation in RAA and LVS occurred. Using SIP antibodies, a positive correlation of protein deposition levels in RAA and corresponding LVS (r = 0.818) could be shown for ED-A+ Fn. Cardiac tissue remodelling is likely a process involving the entire heart reflected by intra-individually comparable histology and cECM changes in RAA and LVS samples. ED-A+ Fn might be an excellent target for an antibody-mediated delivery of diagnostic or therapeutic agents. The RAA is a valuable and representative tool to evaluate cardiac remodelling and to plan individualized therapy.

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Acknowledgments

The authors would like to thank Claudia Seliger for excellent technical assistance. The research leading to the results has received funding from the Friedrich Schiller University of Jena and from the European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement no. Health-F2-2008-201342 (ADAMANT).

Conflict of interest

Dario Neri is a co-founder and shareholder of Philogen, the company which owns the rights over the F8 and the F16 antibody.

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Authors and Affiliations

  1. Department of Internal Medicine I, University Hospital of Jena, Erlanger Allee 101, 07740, Jena, Germany

    Anja Baldinger, Bernhard R. Brehm, Hans-Reiner Figulla & Marcus Franz

  2. Institute of Pathology, University Hospital Jena, Jena, Germany

    Petra Richter & Alexander Berndt

  3. Department of Cardiothoracic Surgery, University Hospital of Jena, Jena, Germany

    Torsten Bossert, Katja Gruen & Khosro Hekmat

  4. Institute of Pathology, HELIOS-Klinikum Erfurt, Erfurt, Germany

    Hartwig Kosmehl

  5. Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland

    Dario Neri

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  1. Anja Baldinger
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Correspondence to Marcus Franz.

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Baldinger, A., Brehm, B.R., Richter, P. et al. Comparative analysis of oncofetal fibronectin and tenascin-C expression in right atrial auricular and left ventricular human cardiac tissue from patients with coronary artery disease and aortic valve stenosis. Histochem Cell Biol 135, 427–441 (2011). https://doi.org/10.1007/s00418-011-0809-z

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