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Effect of amino acid sequence variations at position 149 on the fusogenic activity of the subtype B avian metapneumovirus fusion protein

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Abstract

The entry of enveloped viruses into host cells requires the fusion of viral and cell membranes. These membrane fusion reactions are mediated by virus-encoded glycoproteins. In the case of avian metapneumovirus (aMPV), the fusion (F) protein alone can mediate virus entry and induce syncytium formation in vitro. To investigate the fusogenic activity of the aMPV F protein, we compared the fusogenic activities of three subtypes of aMPV F proteins using a TCSD50 assay developed in this study. Interestingly, we found that the F protein of aMPV subtype B (aMPV/B) strain VCO3/60616 (aMPV/vB) was hyperfusogenic when compared with F proteins of aMPV/B strain aMPV/f (aMPV/fB), aMPV subtype A (aMPV/A), and aMPV subtype C (aMPV/C). We then further demonstrated that the amino acid (aa) residue 149F contributed to the hyperfusogenic activity of the aMPV/vB F protein. Moreover, we revealed that residue 149F had no effect on the fusogenic activities of aMPV/A, aMPV/C, and human metapneumovirus (hMPV) F proteins. Collectively, we provide the first evidence that the amino acid at position 149 affects the fusogenic activity of the aMPV/B F protein, and our findings will provide new insights into the fusogenic mechanism of this protein.

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Acknowledgments

This work was supported by the Fund for the Modern Agro-Industry Technology Research System (no. nycytx-42-G3-01). We are grateful to Dr. Pinghuang Liu for critical revision of the manuscript.

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Authors and Affiliations

  1. Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nan Gang District, Harbin, 150001, Heilongjiang, People’s Republic of China

    Bingling Yun, Yanni Gao, Yongzhen Liu, Xiaolu Guan, Yongqiang Wang, Xiaole Qi, Honglei Gao, Changjun Liu, Hongyu Cui, Yanping Zhang, Yulong Gao & Xiaomei Wang

  2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, 225009, People’s Republic of China

    Xiaomei Wang

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  1. Bingling Yun
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Yun, B., Gao, Y., Liu, Y. et al. Effect of amino acid sequence variations at position 149 on the fusogenic activity of the subtype B avian metapneumovirus fusion protein. Arch Virol 160, 2445–2453 (2015). https://doi.org/10.1007/s00705-015-2524-x

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  • DOI: https://doi.org/10.1007/s00705-015-2524-x

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