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Biogenic synthesis of silver nanoparticles using rhizome extract of Dysosma pleiantha and its antiproliferative effect against breast and human gastric cancer cells

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Abstract

Synthesis of biogenic metal nanoparticles using plant extract has gained considerable attention in recent years. The present study aims to synthesize and investigate the cytotoxic effect of silver nanoparticles (AgNPs) from Dysosma pleiantha rhizome extract. The green biosynthesis of AgNPs was verified by ultraviolet visible spectrometer, and characterized using fourier transform infrared spectroscopy, transmission electron microscopy and scanning electron microscopy. Results of microscopic studies revealed that the synthesized AgNPs were a spherical shape with an average size of 76 nm. We also examined the anti-cancer activity of biologically synthesized AgNPs. The dose-dependent cytotoxicity was observed in the breast cancer cell lines MDA-MB-231 and MDA-MB-453 treated with biogenically synthesized AgNPs, and the IC50 was recorded at 33.521  and 36.25 µM respectively. The DNA fragmentation analysis showed that the MDA-MB-231 cells treated with increasing concentrations of AgNPs significantly triggered the fragmentation of DNA. In addition, the synthesized AgNPs exhibited dose dependent cytotoxic potential against human gastric cancer cell lines and the IC50 was recorded at 7.14 µM. Thus, the green biosynthesized AgNPs from D. pleiantha rhizome can be used in the novel development of anticancer drugs.

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Acknowledgements

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. First author wish to thank Dr. K.J. Senthil Kumar for his kind help during the cell culture analysis.

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Authors and Affiliations

  1. Department of Applied Chemistry, Chaoyang University of Technology, Taichung, 41349, Taiwan, ROC

    Palaniyandi Karuppaiya & Hsin Sheng Tsay

  2. Department of Chemistry, National Chung Hsing University, Taichung, 40249, Taiwan, ROC

    Elumalai Satheeshkumar

  3. Department of Agronomy, National Chung Hsing University, Taichung, 40249, Taiwan, ROC

    Hsin Sheng Tsay

Authors
  1. Palaniyandi Karuppaiya
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  2. Elumalai Satheeshkumar
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  3. Hsin Sheng Tsay
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Contributions

PK performed the laboratory experiments and collected the data. PK and SE performed UV–visible Spectrophotometer and FTIR analysis. PK and HST interpreted the data, drafted and revised the manuscript. PK and HST designed and supervised the study. All authors read and approved the final version of the manuscript.

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Correspondence to Palaniyandi Karuppaiya or Hsin Sheng Tsay.

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Karuppaiya, P., Satheeshkumar, E. & Tsay, H.S. Biogenic synthesis of silver nanoparticles using rhizome extract of Dysosma pleiantha and its antiproliferative effect against breast and human gastric cancer cells. Mol Biol Rep 46, 4725–4734 (2019). https://doi.org/10.1007/s11033-019-04917-1

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  • DOI: https://doi.org/10.1007/s11033-019-04917-1

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