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Maize cytosolic NADP-malic enzyme (ZmCytNADP-ME): a phylogenetically distant isoform specifically expressed in embryo and emerging roots

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Abstract

Two maize plastidic NADP-malic enzyme isoforms have been characterized: the bundle sheath-located photosynthetic isoform (ZmC4-NADP-ME) and a constitutively expressed one (Zm-nonC4-NADP-ME). In this work, the characterization of the first maize cytosolic NADP-ME (ZmCytNADP-ME) is presented, which transcript is exclusively found in embryo and emerging roots. ZmCytNADP-ME expression in roots decreases with development, while Zm-nonC 4 -NADP-ME increases concomitantly. On the other hand, ZmCytNADP-ME accumulation is differentially modulated by several stress conditions and shows coordination with that of Zm-nonC 4 -NADP-ME in maize young roots. Recombinant ZmCytNADP-ME displays clearly distinct kinetic parameters and metabolic regulation than the plastidic isoforms. The particular properties and the specific-expression pattern of this novel isoform suggest that it may be involved in the control of cytosolic malate levels in emerging roots, e.g. during hypoxia. ZmCytNADP-ME is phylogenetically related to other cytosolic mono and dicot NADP-MEs, and data indicate that it belongs to an ancestral unique group among plant NADP-MEs.

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Acknowledgments

This work was funded by grants from CONICET and Agencia Nacional de Promoción Científica y Tecnológica to MFD and CSA. Further support was provided by the Deutsche Forschungsgemeinschaft to VGM. MFD and CSA are members of the Researcher Career of CONICET. CA and GM are fellows of the same institution.

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Authors and Affiliations

  1. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina

    Enrique Detarsio, Clarisa E. Alvarez, Gabriela L. Müller, Carlos S. Andreo & María F. Drincovich

  2. Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, 50931, Cologne, Germany

    Verónica G. Maurino

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  1. Enrique Detarsio
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  2. Verónica G. Maurino
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  3. Clarisa E. Alvarez
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  4. Gabriela L. Müller
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  5. Carlos S. Andreo
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  6. María F. Drincovich
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Corresponding author

Correspondence to María F. Drincovich.

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Enrique Detarsio and Verónica G. Maurino contributed equally to this work.

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Detarsio, E., Maurino, V.G., Alvarez, C.E. et al. Maize cytosolic NADP-malic enzyme (ZmCytNADP-ME): a phylogenetically distant isoform specifically expressed in embryo and emerging roots. Plant Mol Biol 68, 355–367 (2008). https://doi.org/10.1007/s11103-008-9375-8

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