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Optimization of cell/tissue culture of Linum persicum for production of lignans derivatives including Podophyllotoxin

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A Correction to this article was published on 07 February 2018

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Abstract

Linum persicum contains significant amount of lignans as podophyllotoxin that may show therapeutic effects against several types of metastatic cancers such as breast cancer. The aim of this study was to establish the cell culture of L. persicum for lignans production and evaluation for anti-carcinogenic activity on MCF7 cell line. Also, lignan contents of the cell, callus, and plantlets and also in different parts including; capsule, leave, stem and root of the wild plants were determined using HPLC technique. The highest podophyllotoxin in capsules and pinoresinol in leaf and root organs were observed. Shoot extract of the in-vitro plantlets have greater cytotoxic effect on breast cancer cells (IC50 = 5 µg/ml) and leads to significant raise in ratio of Bax/Bcl-2 value = 1.42 and Rbl1 gene expression that are well-known as apoptotic inducing agents. In conclusion, due to cytotoxic activity of in vitro plantlet extracts on MCF7 cell line with limited side-effects; the corresponding extracted component could be led to therapeutic approaches in future.

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Change history

  • 07 February 2018

    There was an spelling error in the seventh author’s first name in the initial online publication. The original article has been corrected.

Abbreviations

PTOX:

Podophyllotoxin

FW:

Fresh weight

MPTOX:

Methoxy podophyllotoxin

NAA:

1-Naphthalene acetic acid

Kin:

Kinetin

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Acknowledgements

This work was supported financially by Tarbiat Modares University and Stem Cell Technology Research Center.

Author information

Authors and Affiliations

  1. Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, 14115-154, Iran

    Mina Esfandiari & Mohsen Sharifi

  2. Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran, Iran

    Mina Esfandiari

  3. Department of Chemical Engineering, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran

    Farzaneh Mohamadyar-Toupkanlou

  4. Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran

    Hana Hanaee-Ahwaz

  5. Department of Marine Biology, Hormozgan University, Bandar Abbas, Iran

    Morteza Yousefzadi

  6. Department of Phytochemistry, Faculty of Science, Yasouj University, Yasouj, Iran

    Azizollah Jafari

  7. Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Simzar Hosseinzadeh

  8. Department of Hematology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran

    Masoud Soleimani

Authors
  1. Mina Esfandiari
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  2. Mohsen Sharifi
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  3. Farzaneh Mohamadyar-Toupkanlou
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  4. Hana Hanaee-Ahwaz
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  5. Morteza Yousefzadi
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  6. Azizollah Jafari
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  7. Simzar Hosseinzadeh
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  8. Masoud Soleimani
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Contributions

This research paper was accomplished with the collaboration of authors. ME performed the experiments, analyzed data and wrote the manuscript. MS designed the study, supervised the study and wrote the manuscript. FM-T analyzed data. HH-A wrote the manuscript. MY advised the study. AJ collected plant samples. SH and MS advised the study and edited the manuscript.

Corresponding author

Correspondence to Mohsen Sharifi.

Additional information

Communicated by Sergio J. Ochatt.

The original version of this article has been revised: The seventh author's first name has been corrected.

A correction to this article is available online at https://doi.org/10.1007/s11240-018-1386-9.

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Esfandiari, M., Sharifi, M., Mohamadyar-Toupkanlou, F. et al. Optimization of cell/tissue culture of Linum persicum for production of lignans derivatives including Podophyllotoxin. Plant Cell Tiss Organ Cult 133, 51–61 (2018). https://doi.org/10.1007/s11240-017-1360-y

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  • DOI: https://doi.org/10.1007/s11240-017-1360-y

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