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Diet-induced obesity, exogenous leptin-, and MADB106 tumor cell challenge affect tissue leukocyte distribution and serum levels of cytokines in F344 rats

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Abstract

The adipocyte-derived catabolic protein leptin alters cell-mediated immunity and cytokine crosstalk. This may provide new insights into the altered immune response, seen in obese individuals. Therefore, we determined the tissue distribution of immune cells in diet-induced obese (dio) and normal weight F344 rats challenged with MADB106 tumor cells or leptin. Immune cell distribution in blood (by FACS analysis) and tissues (NK cells in spleen and liver, immunohistologically) as well as pro-inflammatory cytokines (IL-6, TNF-α; by flow cytometry) were investigated in 28 normal weight and 28 dio rats (n = 4–6/group). Pro-inflammatory cytokines were increased 3-fold for IL-6 and 7-fold for TNF-α in obese animals. Higher numbers of blood monocytes and NK cells were found in obese as compared to normal weight animals. In dio rats challenged with leptin and MADB106 tumor cells, monocyte numbers were decreased as compared to the obese control animals. Immunohistochemistry revealed an altered NK cell distribution in a compartment-, treatment-, and bodyweight-specific manner. In conclusion, our data reveal a distinct distribution pattern of monocytes and NK cells in dio rats as compared to normal weight littermates and an additional modulatory effect of a leptin- and MADB106 tumor cell challenge.

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Acknowledgments

The excellent technical assistance of I. Dressendörfer, S. Faßbender, and S. Kuhlmann is very much appreciated. We thank J. Schade for her help with the MADB106 cell staining. This study was supported by a grant from the Danone Foundation, Haar, Germany.

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Authors and Affiliations

  1. Institute for Functional and Applied Anatomy, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany

    Patrick Behrendt, Tobias Buchenauer, Felix Bode & Heike Nave

  2. Clinic for Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany

    Rüdiger Horn

  3. Department of Endocrinology, Christie Hospital, Manchester, UK

    Georg Brabant

  4. Clinic for Clinical Immunology and Rheumatology, Hannover Medical School, Hannover, Germany

    Roland Jacobs

  5. Clinic for Psychosomatics and Psychotherapy, Hannover Medical School, Hannover, Germany

    Michael Stephan

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  1. Patrick Behrendt
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Correspondence to Heike Nave.

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Behrendt, P., Buchenauer, T., Horn, R. et al. Diet-induced obesity, exogenous leptin-, and MADB106 tumor cell challenge affect tissue leukocyte distribution and serum levels of cytokines in F344 rats. Endocr 38, 104–112 (2010). https://doi.org/10.1007/s12020-010-9358-9

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  • DOI: https://doi.org/10.1007/s12020-010-9358-9

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