Abstract
Microorganisms are essential to maintain ecosystem functions and health as they catalyze most keystone processes. Formerly the quantification of key functions was performed by broad ranged primers, which were mainly based on genomes of cultivated microbes. To take advantage of the enormous development of next generation sequencing technologies, we propose to use metagenomic data for the design of new primer systems. Here we describe a two-phasic approach for a targeted and site specific primer design for qPCR.
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Fabian Bergkemper, Stefanie Schulz, Maria de Vries, Anne Schöler und Michael Schloter (v. l. n. r.)
Die Abteilung für Umweltgenomik unter der Leitung von Prof. Dr. Michael Schloter untersucht Mikrobiome in unterschiedlichen Umweltkompartimenten und versucht aus den Daten ökologische Gesetzmäßigkeiten der Interaktionsmuster abzuleiten. Im Fokus sind dabei Interaktionen zwischen Mikroorganismen und dem Menschen (AG Dr. Anne Schöler) und der Pflanze (AG Prof. Dr. Schröder) sowie Mikrobiomstrukturen in Böden (AG Dr. Stefanie Schulz).
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Schulz, S., Bergkemper, F., De Vries, M. et al. qPCR zur quantitativen Validierung von Metagenomdaten. Biospektrum 22, 265–269 (2016). https://doi.org/10.1007/s12268-016-0684-1
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DOI: https://doi.org/10.1007/s12268-016-0684-1