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HCS-Mikroskopie – ein Schlüssel zu intrazellulären Schmerzmechanismen

  • Wissenschaft · Special: High Content Imaging
  • Imaging in der medizinischen Forschung
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BIOspektrum Aims and scope

Abstract

Pain sensitivity is strongly modulated by intracellular signalling. The analysis of pain signalling is hampered by small numbers of neurons, high cellular heterogeneity, and near absence of transfectability. HCS microscopy allowed us to proof that nociceptive neurons express specific signalling components, to analyze signalling activity of endogenous signalling components with single cell resolution, and thereby to suggest blockers of Nav1.7 as novel therapeutic approach to boost the efficacy of opioids.

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Literatur

  1. Isensee J, Diskar M, Waldherr S et al. (2014) Pain modulators regulate the dynamics of PKA-RII phosphorylation in subgroups of sensory neurons. J Cell Sci 127:216–229

    Article  CAS  PubMed  Google Scholar 

  2. Andres C, Hasenauer J, Allgower F et al. (2012) Thresholdfree population analysis identifies larger DRG neurons to respond stronger to NGF stimulation. PLoS One 7:e34257

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Isensee J, Wenzel C, Buschow R et al. (2014) Subgroupelimination transcriptomics identifies signaling proteins that define subclasses of TRPV1-positive neurons and a novel paracrine circuit. PLoS One 9:e115731

    Article  PubMed  PubMed Central  Google Scholar 

  4. Isensee J, Krahé L, Moeller K et al. (2017) Synergistic regulation of serotonin and opioid signaling contribute to pain insensitivity in Nav1.7 knockout mice. Sci Signal 10, doi: 10.1126/scisignal.aah4874

    Google Scholar 

  5. Hucho T, Suckow V, Joseph EK et al. (2012) Ca++/CaMKII switches nociceptor-sensitizing stimuli into desensitizing stimuli. J Neurochem 123:589–601

    Article  CAS  PubMed  Google Scholar 

  6. Hucho T (2014) [1 + 1 = 0. Reprogramming of nociceptors]. Schmerz 28:628–630

    Article  CAS  PubMed  Google Scholar 

  7. Deuis JR, Dekan Z, Wingerd JS et al. (2017) Pharmacological characterisation of the highly NaV1.7 selective spider venom peptide Pn3a. Sci Rep 7:40883

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Experimentelle Anästhesiologie und Schmerzforschung, Klinik für Anästhesiologie und Operative Intensivmedizin, Universitätsklinikum Köln (AöR), Robert-Koch-Straße 10, D-50931, Köln, Deutschland

    Jörg Isensee & Tim Hucho

Authors
  1. Jörg Isensee
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  2. Tim Hucho
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Correspondence to Tim Hucho.

Additional information

Jörg Isensee 1997–2002 Biotechnologiestudium an der Hochschule für Angewandte Wissenschaften, Hamburg. 2002–2004 Studium der Life Sciences an der Universität Hannover. 2004–2009 Promotion an der Charité, Berlin. 2009–2012 Postdoc am Max-Planck-Institut für molekulare Genetik, Berlin. Seit 2012 Postdoc in der Uniklinik Köln.

Tim Hucho 1991–1997 Biochemiestudium an der Universität Leipzig und der FU Berlin. 1997–2002 Promotion bei Prof. Dr. Y. A. Barde am Max-Planck-Institut für Neurobiologie, Martinsried. 2002–2005 Postdoc bei Prof. Dr. J. D. Levine an der University of California, San Francisco, USA. 2005–2012 AG-Leiter bei Prof. Dr. H. H. Ropers am Max-Planck-Institut für molekulare Genetik, Berlin. Seit 2012 W2-Professor für Klinische und Experimentelle Anästhesiologie an der Universität zu Köln.

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Isensee, J., Hucho, T. HCS-Mikroskopie – ein Schlüssel zu intrazellulären Schmerzmechanismen. Biospektrum 23, 277–280 (2017). https://doi.org/10.1007/s12268-017-0796-2

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  • DOI: https://doi.org/10.1007/s12268-017-0796-2

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