Abstract
Single-cell RNA-seq has revolutionized our understanding of cellular heterogeneity in a wide spectrum of biological processes. Pioneering studies suggest complex mechanisms and division of labour employed by the bacterial pathogen Salmonella to subvert host cell functions during infection. Here we describe some of our findings from single-cell RNA-seq studies that have revealed and characterized heterogeneity between individual cells infected with this intracellular pathogen, and outline future challenges in this burgeoning field.
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Antoine-Emmanuel Saliba 2000–2005 Biotechnologie- und Biochemiestudium am Institut National des Sciences Appliquées (INSA), Toulouse, Frankreich. 2005–2009 Promotion am Institut Curie, Paris, Frankreich. 2009–2013 EMBL Interdisciplinary Postdoc(EIPOD)-Stipendiat am EMBL in Heidelberg, 2013–2016 Postdoc in der Arbeitsgruppe von Prof. Dr. J. Vogel an der Universität Würzburg. Seit 2017 Gruppenleiter am Helmholtz-Institut für RNA-basierte Infektionsforschung (HIRI) in Würzburg.
Alexander J. Westermann 2005–2010 Studium der Molecular Biosciences an der Universität Heidelberg. 2009 Austauschwissenschaftler an der University of California, Berkeley, USA. 2010–2015 Stipendiat des Bayerischen Elitenetzwerks in der Arbeitsgruppe von Prof. Dr. J. Vogel an der Universität Würzburg, dort seit 2015 Postdoc.
Jörg Vogel 1996–1999 Promotion an der HU Berlin und dem Imperial College, London, UK. 2000–2001 Postdoc an der Universität Uppsala, Schweden. 2002–2003 EMBO-Stipendiat an der Hebrew University, Jerusalem, Israel. 2004–2010 Leiter der Nachwuchsgruppe „RNA-Biologie“ am Max-Planck-Institut für Infektionsbiologie, Berlin. Seit 2009 Direktor und W3-Professor des Instituts für Molekulare Infektionsbiologie der Universität Würzburg und zusätzlich seit 2017 Gründungsdirektor des Helmholtz-Instituts für RNA-basierte Infektionsforschung (HIRI) in Würzburg.
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Saliba, AE., Westermann, A.J. & Vogel, J. Einzelzell-RNA-Sequenzierung beleuchtet den Infektionsprozess. Biospektrum 23, 525–528 (2017). https://doi.org/10.1007/s12268-017-0836-y
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DOI: https://doi.org/10.1007/s12268-017-0836-y