Short communicationDifferent blocking agents cause variation in the immunologic detection of proteins transferred to nitrocellulose membranes
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Chapter 33 Performing and Optimizing Western Blots with an Emphasis on Chemiluminescent Detection
2009, Methods in EnzymologyCitation Excerpt :Many different blocking reagents are available for Western blotting. Because no blocking reagent is appropriate for all systems, empirical testing is essential (Spinola and Cannon, 1985). An optimal blocking buffer maximizes the signal-to-noise ratio and does not react with the system's antibodies or target.
Western blotting
2006, MethodsBlocking nonspecific adsorption of native food-borne microorganisms by immunomagnetic beads with ι-carrageenan
2004, Carbohydrate ResearchCitation Excerpt :Because of the above, we were interested in determining if any blocking agents17 exist that might ameliorate the nonspecific adhesion of bacteria onto IMBs and other capture surfaces. Most blocking agents have been developed for immunoassay applications18–22 and are made from either nonspecific sera, detergents, milk proteins, bovine serum albumin (BSA), ovalbumin, gelatin, or some mixture derived therefrom.17,21,23 These blocking reagents increase an immunoassay’s signal-to-noise ratio by interacting with all solid-phase components and ‘quenching’20 the nonspecific binding of reporter antibodies onto these surfaces.
A solid-phase method for evaluation of gold conjugate used in quantitative detection of antigen by immunogold-labeling electron microscopy
2003, Journal of Immunological MethodsProtein blotting: A review
2003, Journal of Immunological MethodsThe use of Tween 20 in immunoblotting assays for the detection of autoantibodies in connective tissue diseases
2000, Journal of Immunological Methods