Biochemical and Biophysical Research Communications
Impact of persistent cytomegalovirus infection on human neuroblastoma cell gene expression
Section snippets
Materials and methods
Cell cultures and virus strains. Human NB cell line MHH-NB-11 was obtained by the “Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH” (DSMZ, Braunschweig, Germany). The human NB cell lines UKF-NB-2, UKF-NB-3, and UKF-NB-4 were established as described before [12]. SH-SY5Y was obtained from ATTC/LGC (Promochem, Wesel, Germany). All cells were propagated in Iscove’s modified Dulbecco’s medium (IMDM, Seromed, Berlin, Germany) supplemented with 10% fetal calf serum (FCS, Gibco, Karlsruhe,
Alteration in gene expression by productive persistent infection of NB cells
A microarray approach was applied to assess changes in host cell gene expression in response to persistent HCMV infection. Focusing on the general impact of persistent HCMV infection on neuroblastoma cell lines we compared the gene expression of several parental NB cell lines (MHH-NB-11, UKF-NB-4, and UKF-NB-2) to their persistently AD169 or Hi91-infected sub-cell lines (MHH-NB-11AD169, UKF-NB-2AD169, UKF-NB-4AD169, MHH-NB-11Hi91, and UKF-NB-4Hi91). Applying strict filter algorithms followed by
Acknowledgments
This work was supported by the friendly organization “Hilfe für krebskranke Kinder Frankfurt e.V.” and its foundation “Frankfurter Stiftung für krebskranke Kinder.” The authors wish to express their sincere thanks Rouslan Kotchetkov for fruitful discussion, to Lena Stegmann and Gesa Meincke for their skillful technical assistance.
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These authors contributed equally to this paper.