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Temperature- and solvent-tolerant mutants of filamentous bacteriophage helper M13 KO7

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Abstract

Mutants of helper phage M13 KO7 were isolated whose survival was up to 300-fold greater than the wild type after 30 min at 95 °C, and had increased solvent tolerance (up to 200-fold in 75% v/v N,N-dimethylformamide). These mutants retained their ability to package pIII-fusion display phagemids with normal efficiency and therefore will be useful in the isolation of stable protein variants by phage display.

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Authors and Affiliations

  1. Department of Biology and Biochemistry and 2Department of Chemical Engineering, University of Houston, Houston, TX , 77204-5513, USA

    Robert R. Riddle, Richard C. Willson & Michael J. Benedik

  2. Department of Chemical Engineering, University of Houston, Houston, TX , 77204-5513, USA

    Richard C. Willson

Authors
  1. Robert R. Riddle
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  2. Richard C. Willson
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  3. Michael J. Benedik
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Riddle, R.R., Willson, R.C. & Benedik, M.J. Temperature- and solvent-tolerant mutants of filamentous bacteriophage helper M13 KO7. Biotechnology Letters 21, 87–90 (1999). https://doi.org/10.1023/A:1005402209276

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  • DOI: https://doi.org/10.1023/A:1005402209276

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