Abstract
Segmentation of the Drosophila embryo is established at around the blastoderm stage1–3 and requires both maternal information in the egg cytoplasm4,5 and expression of the zygotic genome. Zygotic genes involved in segmentation have been defined by mutations that affect the segmentai pattern of the embryo6–10, and these fall into several different classes, such as the 'gap' genes, mutations of which cause the loss of contiguous segments6. Krüppel (Kr) is an example of a Drosophila gap genes, strong Kr mutant embryos lacking all thoracic and five anterior abdominal segments, with part of the remaining posterior abdominal segments being present as a mirror-image duplication (weaker alleles cause shorter deletions11,12). Kr has been cloned12 and shown to encode a blas-toderm-gastrulation stage-specific transcript expressed in regions of the embryo affected by Kr mutants13. We report here that the protein product of the Kr gene predicted from DNA sequences is structurally homologous to the transcription factor TFIIIA which regulates 5S gene expression in Xenopus. Like TFIIIA, the predicted Kr protein has an internally repetitious sequence, and as has been suggested for TFIIIA, the repeat units may form DNA-binding domains.
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References
Wieschaus, E. & Gehring, W. Devl Biol. 50, 249–263 (1976).
Szabad, J., Schüpbach, T. & Wieschaus, E. Devl Biol. 73, 265–271 (1979).
Simcox, A. A. & Sang, J. H. Devl Biol. 97, 212–221 (1983).
Nüsslein-Volhard, C. in Determinants of Spatial Organisation (eds Subtelney, S. & Königsberg, I. R.) 185–211 (Academic, New York, 1979).
Schubiger, G., Moseley, R. C. & Wood, W. J. Proc. natn. Acad. Sci. U.S.A. 74, 2050–2053 (1977).
Nüsslein-Volhard, C. & Wieschaus, E. Nature 287, 283–295 (1980).
Wieschaus, E., Nüsslein-Volhard, C. & Jürgens, G. Wilhelm Roux Arch. dev. Biol. 193, 296–307 (1984).
Nüsslein-Volhard, C., Wieschaus, E. & Kluding, H. Wilhelm Roux Arch. dev. Biol. 193, 267–282 (1984).
Jürgens, G., Kluding;, H., Nüsslein-Volhard, C. & Wieschaus, E. Wilhelm Roux Arch. dev. Biol. 193, 283–295 (1984).
Nüsslein-Volhard, C., Wieschaus, E. & Jürgens, G. Verh. dt. zool. Ges., 91–104 (1982).
Wieschaus, E., Nüsslein-Volhard, C. & Kluding, G. Devl Biol. 104, 172–186 (1984).
Preiss, A., Rosenberg, U. B., Kienlin, A., Seifert, E. & Jäckle, H. Nature 313, 27–32 (1985).
Knipple, D. C. et al. Nature 317, 40–44 (1985).
Kozak, M. Nucleic Acids Res. 12, 857–872 (1984).
Laughton, A. & Scott, M. P. Nature 310, 25–31 (1984).
Poole, S., Kauvar, L. M., Drees, B. & Kornberg, T. Cell 40, 37–43 (1985).
Marshall, R. D. A. Rev. Biochem. 41, 673–702 (1972).
Miller, J., McLachlan, A. D. & Klug, A. EMBO J. 4, 1609–1614 (1985).
Pelham, H. R. B. & Brown, D. D. Proc. natn. Acad. Sci. U.S.A. 77, 4170–4174 (1980).
Hoda, B. W. & Roeder, R. G. Cell 22, 119–126 (1980).
Ohlendorf, D. H. & Matthews, B. W. A. Rev. Biophys. Bioengng 12, 259–284 (1983).
McGinnis, W., Levine, M., Hafen, E., Kuroiwa, A. & Gehring, W. J. Nature 308, 428–433 (1984).
McGinnis, W., Garber, R. L., Wirz, J., Kuroiwa, A. & Gehring, W. J. Cell 37, 403–408 (1984).
Carrasco, A. E., McGinnis, W., Gehring, W. J. & De Robertis, E. M. Cell 37, 409–414 (1984).
Shepherd, J. C. W., McGinnis, W., Carrasco, A. E., De Robertis, E. M. & Gehring, W. J. Nature 310, 70–71 (1984).
Levine, M., Rubin, G. & Tjian, R. Cell 38, 667–673 (1984).
Fjose, A., McGinnis, W. J. & Gehring, W. J. Nature 313, 284–289 (1985).
Meinhard, H. Ber. Bunsenges. phys. Chem. 89, 691–699 (1985).
Meinhard, H. J. Cell Sci. (in the press).
Dyan, W. S. & Tjian, R. Nature 316, 774–777 (1985).
Kuroiwa, A., Hafen, E. & Gehring, W. J. Cell 37, 825–831 (1984).
Vincent, A., O'Connell, P., Gray, M. R. & Rosbash, M. EMBO J. 3, 1003–1013 (1984).
Vincent, A., Colot, H. V. & Rosbash, M. J. molec. Biol. 186, 149–166 (1985).
Hood, L. M., Campbell, J. H. & Elgin, S. C. R. A. Rev. Genet. 9, 305–334 (1975).
Brown, R. S., Sander, C. & Argos, P. FEBS Lett. 186, 271–274 (1985).
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Rosenberg, U., Schröder, C., Preiss, A. et al. Structural homology of the product of the Drosophila Krüppel gene with Xenopus transcription factor IIIA. Nature 319, 336–339 (1986). https://doi.org/10.1038/319336a0
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DOI: https://doi.org/10.1038/319336a0
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