Abstract
Embryonic stem cells offer unprecedented opportunities for random or targeted genome alterations in the mouse. We present here an efficient strategy to create chromosome-specific loss of heterozygosity in embryonic stem cells. The combination of this method with genome-wide mutagenesis in ES cells (using chemical mutagens or gene-trap vectors) opens up the possibility for in vitro or in vivo functional screening of recessive mutations in the mouse.
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Acknowledgements
We thank L. Miquerol for the Vegf knock-in cells, and J. Rossant and members of her lab for the targeted insertions on MMU2, MMU5 and MMU10. This work was supported by a CIHR Centennial Fellowship to L.L. and a grant from CIHR to A.N.
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Lefebvre, L., Dionne, N., Karaskova, J. et al. Selection for transgene homozygosity in embryonic stem cells results in extensive loss of heterozygosity. Nat Genet 27, 257–258 (2001). https://doi.org/10.1038/85808
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DOI: https://doi.org/10.1038/85808
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