Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

Lukas Lomb et al.
Phys. Rev. B 84, 214111 – Published 20 December 2011
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Abstract

X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.

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  • Received 23 July 2011

DOI:https://doi.org/10.1103/PhysRevB.84.214111

©2011 American Physical Society

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Vol. 84, Iss. 21 — 1 December 2011

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