The Legionella pneumophila genome evolved to accommodate multiple regulatory mechanisms controlled by the CsrA-system
Fig 1
The L. pneumophila csrA- mutant shows a defect in intracellular growth.
The L. pneumophila csrA- mutant has a defect in replication in Acanthamoeba castellanii. Grey, wt L. pneumophila strain Paris; green, ΔletA-mutant; Blue, ΔrsmYZ double mutant; Red, csrA—mutant. Infections were performed at 37°C and the number of intracellular, viable bacteria was determined by the standard plate count assay. Each time point represents the mean +/- SD of three biological replicates.