Pathogenic Chlamydia Lack a Classical Sacculus but Synthesize a Narrow, Mid-cell Peptidoglycan Ring, Regulated by MreB, for Cell Division
Fig 2
Structured illumination microscopy of DAAD PG labeling in pathogenic Chlamydia.
Structured illumination microscopy (SIM) was conducted on four pathogenic Chlamydia species. EDA-DA was added 2 hours post infection (hpi) and coverslips were fixed at 18 hpi. PG labeling (represented by EDA—DA) is shown in green, the major C. trachomatis outer membrane protein (MOMP) is shown in red, and cell nuclei are in blue (this labeling scheme is maintained in all subsequent figures, unless otherwise stated). MOMP staining is not shown for the three other species. Images are representative of ~20 inclusions viewed per strain. Scale bar = 1 μm.