Revisiting promyelocytic leukemia protein targeting by human cytomegalovirus immediate-early protein 1
Fig 9
Reduced cytokine and ISG expression during hCMV infection due to lack of IE1-PML interaction.
TetOne cells expressing firefly luciferase and TetOne-IE1 cells expressing the indicated HA-tagged wild-type (wt) or mutant IE1 proteins were treated with dox for 48 h and infected with gTBdlIE1 at an MOI of 2 PFUs/cell for 6 h. Relative mRNA levels were determined by RT-qPCR for the indicated cytokine genes (IFNB1, IFNL1, CCL5, TNF) and PML. Results were normalized to TUBB, and means and standard deviations of three biological and two technical replicates are shown in comparison to cells expressing wt IE1 (set to 1). Statistical significance was assessed in Excel using a two-tailed, unpaired T-test; ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.