Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
Online ISSN : 1882-1006
Print ISSN : 0015-6426
ISSN-L : 0015-6426
Applicability of Reversed-Phase TLC to the Analysis of Coal Tar Dyes in Food
Naoko OZEKI, Hisao OKA, Yoshitomo IKAI, Tsutomu OHNO, Junko HAYAKAWA, Tomoko HAYASHI, Tomihiko AOYAMA, Yasukazu KUSHIBIKI, Takeo SATO, Motome ITO, Ryoji SUZUKI
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Keywords: reversed-phase thin layer chromatography, normal-phase thin layer chromatography, food coal tar dyes
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1993 Volume 34 Issue 6 Pages 542-545_1

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Abstract

In order to investigate the applicability of reversed-phase TLC to the analysis of coal tar dyes in food, 162 foods were analyzed by normal- and reversed-phase thin layer chromatographies (TLC) and the chromatographic behaviors of the dyes were compared. In normal- phase TLC, the spots of dyes were apt to show tailing and smaller Rf values than those of standards due to coexisting substances in samples. However, the separation in reversed-phase TLC was not affected by coexisting substances from food and the spots showed no tailing and always gave the same Rf values as the standards with good reproducibility. These results indicate that the use of reversed-phase TLC makes it easier to identify the dyes in food. Therefore, reversed-phase TLC is considered to be useful technique for the analysis of coal tar dyes in food.

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