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Regulation of targets that are derived from within polycistronic mRNAs

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posted on 2011-12-30, 17:03 authored by Johannes H. Urban, Jörg Vogel
GalT proteins (middle panel). GroEL detection served as loading control (lower panel). () Northern blot of RNA samples taken from strains (grown to an OD of 1) that carry the fusion in combination with the sRNA control plasmid pJV300 (lane 1) or the RyhB expression plasmid (lane 2), or from cells without a fusion plasmid but harboring pJV300 (lane 3) or the RyhB expression plasmid (lane 4). In the upper panel, the blot was probed for the plasmid-expressed fusion mRNA using a labeled dsDNA fragments. In the lower panel, the same blot was hybridized with an -specific probe to detect the chromosomally expressed mRNA. 5 μg of total RNA were loaded in lanes 1 and 2, whereas 20 μg were loaded in lanes 3 and 4.

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