Additional file 1: of Chemical fingerprinting of single glandular trichomes of Cannabis sativa by Coherent anti-Stokes Raman scattering (CARS) microscopy

CARS and two-photon fluorescence (TPF) images in backward and forward direction of secretory cavity of C. sativa var. Bedrobinol (a-d) and C. sativa var. Fedora (e-h) at pump 812.6 nm and Stokes 1064 nm (2861 cm− 1). Green: TPF of chlorophyll a in backward direction (em 560–750 nm) (a, e); White: TPF of organic substances in backward direction (em 380–560 nm) (b, f); Magenta: CARS signal of the essential oil in forward direction (c, g); White: TPF of organic substances in forward direction (d, h). Scale bars 50 μm. (TIF 20253 kb)