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Western blot analysis of the H4K4 acetylation state of cytoplasmic histones

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posted on 2011-12-31, 13:55 authored by T Nicolai Siegel, Taemi Kawahara, Jeffrey A DeGrasse, Christian J Janzen, David Horn, George A M Cross

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Taken from "Acetylation of histone H4K4 is cell cycle regulated and mediated by HAT3 in "

Molecular Microbiology 2008;67(4):762-771.

Published online Jan 2008

PMCID:PMC2253726.

© 2007 The Authors; Journal compilation © 2007 Blackwell Publishing Ltd

Whole-cell lysates (WCL) and cytoplasmic fractions (CF) from up to 2 × 10 cells were analysed with α-H4K4ac, α-H4K4-unmodified, α-enolase and α-Pol 1.

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