Additional file 2: Figure S2. of Prognostic value of protein inhibitor of activated STAT3 in breast cancer patients receiving hormone therapy

Effects of activated signal transducers and activators of transcription 3 (PIAS3) on STAT3 signaling in breast cancer cells. (A) Ectopic PIAS3 overexpression increased expression levels of activated STAT3 (p-STAT3, p-tyr705-STAT3) in estrogen receptor (ER)-positive MCF-7 cells, but decreased those of activated STAT3 in ER-negative MDA-MB-231 cells. (B) Ectopic PIAS3 overexpression attenuated the expression levels of STAT3 downstream genes including cyclin D1, c-myc, Bcl-2, and Mcl-1 in ER-positive MDA-MB-231 cells. However, the expression levels of cyclin D1 and c-myc, but those of Bcl-2 and Mcl-1, were up-regulated in ER-positive breast cancer cells. (PDF 160 kb)