MOESM3 of Temporal transcriptional response to latency reversing agents identifies specific factors regulating HIV-1 viral transcriptional switch

Additional file 3: Fig. S2 Reversal of HIV-1 latency in ACH-2 cells is associated with induction of NT5C3 gene expression. (A) Schematic representation of organization of genes on Chromosome 7 between nucleotides 32916815 to 33606068. (B) ACH-2 cells were treated with prostratin, SAHA or TNF-α and the average fold change in expression of NT5C3 transcripts over time relative to time 0 as evaluated by illumina HT-12 V4 array bead chips was included (N = 2). (C) Western blot analysis of NT5C3 in ACH-2 cells or A3.01 cells post-treatment with SAHA, prostratin or TNF-α. At indicated time points, the cells were washed and lysed. Equal amounts of cell lysate were analyzed by immunoblotting them with anti-NT5C3 or anti-tubulin antibodies. NT5C3 antibody also detects NT5C3L protein.