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Effects of and mutations
figure
posted on 2011-12-30, 17:03 authored by Johannes H. Urban, Jörg VogelCopyright information:
Taken from "Translational control and target recognition by small RNAs "
Nucleic Acids Research 2007;35(3):1018-1037.
Published online 30 Jan 2007
PMCID:PMC1807950.
© 2007 The Author(s).
() Regulation of , and fusions by MicA, MicC and MicF, respectively, in wild-type and an otherwise isogenic strain expressing a truncated RNase E (). Shown are western blots of protein samples that were probed with GFP- and GroEL-specific antibodies as in . Below the western blot panels, northern blots show accumulation of the respective fusion mRNA ( probe), the overexpressed sRNA (from left to right: probing for MicA, MicC or MicF), and 5S rRNA levels as loading control. Samples were taken at an OD of 1. () Accumulation of sRNAs in the absence of Hfq. Shown are northern blots of wild-type (+) and Δ (−) cells that carry the sRNA expression plasmids indicated above each panel in combination with the full-length GFP control plasmid pXG-1. RNA samples were taken at an OD of 1; 5S rRNA probing served as loading control.History
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