Additional file 5: of Chemical fingerprinting of single glandular trichomes of Cannabis sativa by Coherent anti-Stokes Raman scattering (CARS) microscopy

Overlay of a CARS- and two-photon fluorescence (TPF) 3D image of secretory cavity and disk cells of C. sativa var. Bedrobinol. Red: CARS signal at 2907 cm− 1 in forward direction; gray: TPF of organic substances in backward direction (em 380–560 nm); green: TPF of chlorophyll a in backward direction (em 560–750 nm).The colour code was chosen in accordance to the observations made by hyperspectral CARS imaging. (AVI 1751 kb)