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TALENs

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Ralf Kühn

    1. Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

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  2. Wolfgang Wurst

    1. Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany

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  3. Benedikt Wefers

    1. Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1338)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Xanthomonas and the TAL Effectors: Nature’s Molecular Biologist

    • Frank White
    Pages 1-8

  3. TAL Effector DNA-Binding Principles and Specificity

    • Annekatrin Richter, Jana Streubel, Jens Boch
    Pages 9-25

  4. The Development of TALE Nucleases for Biotechnology

    • David G. Ousterout, Charles A. Gersbach
    Pages 27-42

  5. Online Tools for TALEN Design

    • Adam J. Bogdanove, Nicholas J. Booher
    Pages 43-47

  6. Assembly of Customized TAL Effectors Through Advanced ULtiMATE System

    • Junjiao Yang, Shengjie Guo, Pengfei Yuan, Wensheng Wei
    Pages 49-60

  7. Engineering Customized TALENs Using the Platinum Gate TALEN Kit

    • Tetsushi Sakuma, Takashi Yamamoto
    Pages 61-70

  8. Design, Assembly, and Characterization of TALE-Based Transcriptional Activators and Repressors

    • Pratiksha I. Thakore, Charles A. Gersbach
    Pages 71-88

  9. A New Approach to Dissect Nuclear Organization: TALE-Mediated Genome Visualization (TGV)

    • Yusuke Miyanari
    Pages 89-97

  10. TALEN-Induced Translocations in Human Cells

    • Marion Piganeau, Benjamin Renouf, Hind Ghezraoui, Erika Brunet
    Pages 99-117

  11. Mutagenesis in Newts: Protocol for Iberian Ribbed Newts

    • Toshinori Hayashi, Takashi Takeuchi
    Pages 119-126

  12. Targeted Mutagenesis in Bombyx mori Using TALENs

    • Yoko Takasu, Toshiki Tamura, Marian Goldsmith, Michal Zurovec
    Pages 127-142

  13. GeneKnockout by Targeted Mutagenesis in a Hemimetabolous Insect, the Two-Spotted Cricket Gryllus bimaculatus, using TALENs

    • Takahito Watanabe, Sumihare Noji, Taro Mito
    Pages 143-155

  14. Methods for TALEN Evaluation, Use, and Mutation Detection in the Mosquito Aedes aegypti

    • Sanjay Basu, Azadeh Aryan, Mary Etna Haac, Kevin M. Myles, Zach N. Adelman
    Pages 157-177

  15. Methods for TALEN-Mediated Genomic Manipulations in Drosophila

    • Jiyong Liu, Yawen Guo, Changqing Li, Yixu Chen, Renjie Jiao
    Pages 179-190

  16. Targeted Mutagenesis in Zebrafish by TALENs

    • Peng Huang, An Xiao, Xiangjun Tong, Shuo Lin, Bo Zhang
    Pages 191-206

  17. Mutagenesis in Xenopus and Zebrafish using TALENs

    • Yun Liu, Hui Zhao, Christopher H. K. Cheng
    Pages 207-227

  18. Genome Editing in Mice Using TALE Nucleases

    • Benedikt Wefers, Christina Brandl, Oskar Ortiz, Wolfgang Wurst, Ralf Kühn
    Pages 229-243

  19. Genome Editing in Rats Using TALE Nucleases

    • Laurent Tesson, Séverine Remy, Séverine Ménoret, Claire Usal, Reynald Thinard, Chloé Savignard et al.
    Pages 245-259

  20. Designer Nuclease-Mediated Generation of Knockout THP1 Cells

    • Tobias Schmidt, Jonathan L. Schmid-Burgk, Thomas S. Ebert, Moritz M. Gaidt, Veit Hornung
    Pages 261-272
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Keywords

About this book

​This volume provides a comprehensive collection of protocols on new technology across various model organisms.  Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

    Ralf Kühn

  • Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany

    Wolfgang Wurst, Benedikt Wefers

Bibliographic Information

  • Book Title: TALENs

  • Book Subtitle: Methods and Protocols

  • Editors: Ralf Kühn, Wolfgang Wurst, Benedikt Wefers

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2932-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-2931-3Published: 07 October 2015

  • Softcover ISBN: 978-1-4939-4905-2Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2932-0Published: 06 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 274

  • Number of Illustrations: 15 b/w illustrations, 50 illustrations in colour

  • Topics: Human Genetics, Animal Genetics and Genomics, Animal Models

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