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Effects of varied soil nitrogen supply on Norway spruce (Picea abies [L.] Karst.)

II. Carbon metabolism in needles and mycorrhizal roots

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Abstract

The response of carbohydrate metabolism in 3-year-old Norway spruce plants to an increased amount of nitrogen supply to a N-poor forest soil was investigated in a pot experiment. After 7 months of treatment we found a decreased amount of starch in both needles and roots, together with decreased amounts of sucrose in needles of those plants grown under an enhanced inorganic N supply. In addition, the activity and the protein amount of the anaplerotic enzyme phosphoenolpyruvate carboxylase (PEPC) and the activity of NADP-dependent isocitrate dehydrogenase (IDH) were clearly increased. The activity of sucrose phosphate synthase (SPS) and the pool size of fructose 2,6-bisphosphate (F26BP) were not affected by high supply of inorganic N. These data indicate a shift of carbon flow from starch formation towards an enhanced provision of carbon skeletons for N assimilation and shoot growth. In parallel, we found decreased contents of fungus-specific compounds (ergosterol, mannitol, trehalose) in roots, which are indicators of a decreased colonization by ectomycorrhizal fungi, probably as a result of a changed allocation and partitioning of photoassimilates due to an increased N supply.

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Abbreviations

DW:

dry weight

F26BP:

fructose 2,6-bisphosphate

IDH:

NADP-dependent isocitrate dehydrogenase

PEPC:

phosphoenolpyruvate carboxylase, SPS-sucrose phosphate synthase

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Authors and Affiliations

  1. Physiologische Ökologie der Pflanzen, Botanisches Institut, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany

    Thomas Wallenda, Christoph Schaeffer, Werner Einig, Astrid Wingler & Rüdiger Hampp

  2. Institut für Pflanzenernährung, Universität Hohenheim, D-70593, Stuttgart, Germany

    Bettina Seith, Eckhard George & Horst Marschner

Authors
  1. Thomas Wallenda
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  2. Christoph Schaeffer
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  3. Werner Einig
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  4. Astrid Wingler
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  5. Rüdiger Hampp
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  6. Bettina Seith
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  7. Eckhard George
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  8. Horst Marschner
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Deceased 21 September 1996

Deceased 21 September 1996

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Wallenda, T., Schaeffer, C., Einig, W. et al. Effects of varied soil nitrogen supply on Norway spruce (Picea abies [L.] Karst.). Plant Soil 186, 361–369 (1996). https://doi.org/10.1007/BF02415531

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  • DOI: https://doi.org/10.1007/BF02415531

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