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A flow-through microarray cell for the online SERS detection of antibody-captured E. coli bacteria

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Abstract

We present an immunoassay microarray flow-through system for the surface-enhanced Raman scattering (SERS) analysis of bacteria. The system has been constructed to support and automatize the nondestructive in situ analysis of different microorganisms in aqueous environment. After the immobilization of the desired antibodies to an activated PEG-coated surface, the chip is placed into the flow cell which is then flushed with the contaminated sample. Finally, colloidal metal nanoparticles are added and the cells are detected label-free by SERS. Here, we introduce the successful imaging of single microorganisms in the flow cell as well as the quantification of microorganisms in water by SERS mapping with a linear range between 4.3 × 103 to 4.3 × 105 cells/mL. The method has potential for routine application, e.g. for drinking water control.

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Acknowledgments

We would like to thank S. Wiesemann for technical support. A special thanks to M. Wagner for writing an excellent data evaluation program.

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Authors and Affiliations

  1. Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universität München, Marchioninistrasse 17, 81377, Munich, Germany

    Maria Knauer, Natalia P. Ivleva, Reinhard Niessner & Christoph Haisch

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  1. Maria Knauer
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  2. Natalia P. Ivleva
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  3. Reinhard Niessner
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  4. Christoph Haisch
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Corresponding author

Correspondence to Christoph Haisch.

Additional information

Published in the ANAKON special issue with guest editors P. Dittrich, D. Günther, G. Hopfgartner, and R. Zenobi.

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Knauer, M., Ivleva, N.P., Niessner, R. et al. A flow-through microarray cell for the online SERS detection of antibody-captured E. coli bacteria. Anal Bioanal Chem 402, 2663–2667 (2012). https://doi.org/10.1007/s00216-011-5398-0

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  • DOI: https://doi.org/10.1007/s00216-011-5398-0

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