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Genetic diversity and structure among Iranian tall fescue populations based on genomic-SSR and EST-SSR marker analysis

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Abstract

Tall fescue (Festuca arundinacea Schreb. subsp. arundinacea) is one of the most economically important forage grasses in cold and temperate regions of the world. In this study, we have assessed the genetic diversity and structure of wild Iranian tall fescue populations. Thirty-seven individuals from nine natural populations from northern, western, and southern Iranian mountain ranges were analyzed using six genomic-SSRs and five EST-SSRs primer pairs. Our analysis has demonstrated that transcribed EST-SSR regions showed levels of polymorphism similar to genomic-SSR regions. UPGMA, repeated bisection, and model-based Bayesian STRUCTURE clustering methods coupled with neighbor-net network were used to establish six divergent groups of individuals. F ST estimates among clusters showed moderate to low genetic structure. Within-group genetic diversity estimate H and partial correlations between genetic and geographic distances among populations suggested that western Zagros population was related to the rest of the Iranian individuals. The isolation-by-distance hypothesis was not supported by SSR data and the present geographical sampling.

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Acknowledgments

We are grateful to two anonymous reviewers for their critical review of an earlier version of the manuscript. This study was funded by the Agricultural Biotechnology Research Institute of Iran (ABRII). M.S.T. was supported by a doctorate fellowship from the Office of Graduate Studies of the University of Isfahan (Iran). A.D.P. and P.C. were supported by the University of Zaragoza Bioflora research group and BIFI Institute. A.D.P. was also supported by a Central University of Venezuela CDCH PhD fellowship.

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Authors and Affiliations

  1. Department of Biology, University of Shahrekord, Shahrekord, Iran

    Majid Sharifi Tehrani

  2. Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran

    Mohsen Mardi

  3. Department of Biology, University of Isfahan, Isfahan, Iran

    Jamal Sahebi

  4. High Polytechnic School of Huesca, University of Zaragoza, Huesca, Spain

    Pilar Catalán & Antonio Díaz-Pérez

  5. Facultad de Agronomía, Instituto de Genética, Universidad Central de Venezuela, Maracay, Venezuela

    Antonio Díaz-Pérez

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  1. Majid Sharifi Tehrani
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  5. Antonio Díaz-Pérez
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Correspondence to Antonio Díaz-Pérez.

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Sharifi Tehrani, M., Mardi, M., Sahebi, J. et al. Genetic diversity and structure among Iranian tall fescue populations based on genomic-SSR and EST-SSR marker analysis. Plant Syst Evol 282, 57–70 (2009). https://doi.org/10.1007/s00606-009-0207-3

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