Abstract
Resorption lacunae (RL) are discussed as stressors that can increase the risk of mechanical failure in a trabecular network. Quantification of RL has previously been described through the parameter eroded surface/bone surface (ES/BS) as established by light microscopy (LM) analysis, but the results have been inconsistent and contradictory. Using scanning electron microscopy (SEM), a new study design for quantitative evaluation is introduced. To test its applicability a pilot study was executed with trabecular bone dissected from a femoral head of 28 autopsy subjects (14 female and 14 male). A 2.4 × 2.8 × 1.0 mm sample was excised 1.5 cm below the joint surface of each specimen in coronal medial slices of the femoral head and examined. A virtual grid with 1050 squares superimposed over the generated SEM image allowed determination of the ratio of squares containing R_L_ to squares with an u_naffected trabecular surface (RL/U). Classical ES/BS was assessed in parallel sections of the samples. The SEM, and to a lesser extent the qualitative different LM analysis, indicated a gender independent predominance of RL in subjects older than 50 years. This pilot study suggests that the new study design could be useful for acquiring quantitative RL data.
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Gentzsch, C., Junge, M., Pueschel, K. et al. A scanning electron microscopy-based approach to quantify resorption lacunae applied to the trabecular bone of the femoral head. J Bone Miner Metab 23, 205–211 (2005). https://doi.org/10.1007/s00774-004-0585-0
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DOI: https://doi.org/10.1007/s00774-004-0585-0