Abstract
Several transgenic tobacco lines expressing human apolipoprotein A-I (ApoA-I) were obtained. Western blot analyses indicated the expression of the recombinant protein in plant organs at various stages of development, including senescent leaves. A cell line expressing human ApoA-I was established from a T1 transgenic plant. Recombinant ApoA-I was isolated either from extracts of transgenic leaves and from the culture medium of transgenic cells using an antibody-based one-step procedure.
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Acknowledgments
The authors wish to thank Dr Maria Teresa Valente for valuable help in the production of transgenic tobacco plants and Mrs Sara Siervo for technical assistance. This research was supported by grants from the Ministry of University and Research, Italy (PRIN Program 2007), entitled “Plant-based production of recombinant proteins for diagnosis and therapy of human diseases” and “Protein misfolding and amyloid”.
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Purpose of work
Apolipoprotein A-I (ApoA-I), the major component of plasma high-density lipoproteins, plays a pivotal role in preventing atherosclerosis development, being involved in the transport of cholesterol from peripheral cells to the liver where it is degraded. In this study, plants and plant cells cultures are suggested as suitable platforms for the production of ApoA-I for therapeutic or analytical purposes.
Pasquale Chiaiese, Maria Minutolo contributed equally to this work.
An erratum to this article can be found at http://dx.doi.org/10.1007/s10529-010-0441-3
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Chiaiese, P., Minutolo, M., Arciello, A. et al. Expression of human apolipoprotein A-I in Nicotiana tabacum . Biotechnol Lett 33, 159–165 (2011). https://doi.org/10.1007/s10529-010-0388-4
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DOI: https://doi.org/10.1007/s10529-010-0388-4