ABSTRACT
Purpose
This study was conducted to characterize UV imaging as a platform for performing in vitro release studies using Nicorette® nicotine patches as a model drug delivery system.
Methods
The rate of nicotine release from 2 mm diameter patch samples (Nicorette®) into 0.067 M phosphate buffer, pH 7.40, was studied by UV imaging (Actipix SDI300 dissolution imaging system) at 254 nm. The release rates were compared to those obtained using the paddle-over-disk method.
Results
Calibration curves were successfully established which allowed temporally and spatially resolved quantification of nicotine. Release profiles obtained from UV imaging were in qualitative agreement with results from the paddle-over-disk release method.
Conclusion
Visualization as well as quantification of nicotine concentration gradients was achieved by UV imaging in real time. UV imaging has the potential to become an important technology platform for conducting in vitro drug release studies.
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REFERENCES
Clark BC, Dickinson PA, Pyrah IT. In vitro/in vivo release from injectable dispersed systems. In: Burgess DJ, editor. Injectable dispersed systems. Formulation, processing, and performance. Boca Raton: Taylor and Francis; 2005. p. 125–57.
Dutta S, Qiu Y, Samara E, Cao G, Granneman GR. Once-a-day extended-release dosage form of divalproex sodium III: development and validation of a level A in vitro-in vivo correlation (IVIVC). J Pharm Sci. 2005;94:1949–56.
Soto E, Haertter S, Koenen-Bergmann M, Staab A, Troconiz IF. Population in vitro–in vivo correlation model for pramipexole slow-release oral formulations. Pharm Res. 2010;27:340–9.
Takka S, Sakr A, Goldberg A. Development and validation of an in vitro–in vivo correlation for buspirone hydrochloride extended release tablets. J Control Release. 2003;88:147–57.
Larsen C, Larsen SW, Jensen H, Yaghmur A, Østergaard J. Role of in vitro release models in formulation development and quality control of parenteral depots. Expert Opin Drug Deliv. 2009;6:1283–95.
Richardson JC, Bowtell RW, Mäder K, Melia CD. Pharmaceutical applications of magnetic resonance imaging (MRI). Adv Drug Deliv Rev. 2005;57:1191–209.
Kempe S, Metz H, Mäder K. Application of electron paramagnetic resonance (EPR) spectroscopy and imaging in drug delivery research—chances and challenges. Eur J Pharm Biopharm. 2010;74:55–66.
Metz H, Mäder K. Benchtop-NMR and MRI—a new analytical tool in drug delivery research. Int J Pharm. 2008;364:170–5.
Bobiak JP, Koenig JL. Regions of interest in FTIR imaging applications: diffusion of nicotine into ethylene-co-vinyl acetate films. J Control Release. 2005;106:329–38.
Rafferty DW, Koenig JL. FTIR imaging for the characterization of controlled-release drug delivery applications. J Control Release. 2002;83:29–39.
Young PM, Nguyen K, Jones AS, Traini D. Microstructural analysis of porous composite materials: dynamic imaging of drug dissolution and diffusion through porous matrices. AAPS J. 2008;10:560–4.
Zeitler JA, Gladden LF. In vitro tomography and non-destructive imaging at depth of pharmaceutical solid dosage forms. Eur J Pharm Biopharm. 2008;71:2–22.
Olivier J-C, Rabouan S, Couet W. In vitro comparative studies of two marketed transdermal nicotine delivery systems: Nicopatch® and Nicorette®. Int J Pharm. 2003;252:133–40.
Ho H, Chien YW. Kinetic evaluation of transdermal nicotine delivery systems. Drug Dev Ind Pharm. 1993;19:295–313.
Lin S, Ho H, Chien YW. Development of a new nicotine transdermal delivery system: in vitro kinetics studies and clinical pharmacokinetic evaluations in two ethnic groups. J Control Release. 1993;26:175–93.
Baker R. Controlled release of biologically active agents. New York: Wiley; 1987.
Shah VP, Tymes NW, Ment W, Shelly JP. Collaborative study results of a dissolution test procedure developed by FDA for nitroglycerin transdermal delivery systems. Pharmacop Forum. 1988;14:3458–62.
Østergaard J, Jensen H. Simultaneous evaluation of ligand binding properties and protein size by electrophoresis and Taylor dispersion in capillaries. Anal Chem. 2009;81:8644–8.
Higuchi T. Physical chemical analysis of percutaneous absorption process from creams and ointments. J Soc Cosmet Chem. 1960;11:85–97.
Higuchi WI. Analysis of data on the medicament release from ointments. J Pharm Sci. 1962;51:802–4.
Stevens LE, Missel PJ. Impact of density gradients on flow-through dissolution in a cylindrical flow cell. Pharm Dev Technol. 2006;11:529–34.
D’Arcy DM, Liu B, Bradley G, Healy AM, Corrigan OI. Hydrodynamic and species transfer simulations in the USP 4 dissolution apparatus: considerations for dissolution in a low velocity pulsing flow. Pharm Res. 2010;27:246–58.
D’Arcy DM, Corrigan OI, Healy AM. Evaluation of hydrodynamics in the basket dissolution apparatus using computational fluid dynamics-dissolution rate implications. Eur J Pharm Sci. 2006;27:259–67.
ACKNOWLEDGEMENTS
This work was supported by the Danish Medical Research Council. JOE and EML thank LEO Pharma for financial support. JL thanks Yorkshire Forward for support through R&D Grant, project number YHF/02803/RD09.
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Østergaard, J., Meng-Lund, E., Larsen, S.W. et al. Real-Time UV Imaging of Nicotine Release from Transdermal Patch. Pharm Res 27, 2614–2623 (2010). https://doi.org/10.1007/s11095-010-0257-9
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DOI: https://doi.org/10.1007/s11095-010-0257-9