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Soil microbial diversity: an ISO standard for soil DNA extraction

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References

  • Frostegard A, Courtois S, Ramisse V, Clerc S, Bernillon D, Le Gall F et al (1999) Quantification of bias related to the extraction of DNA directly from soils. Appl Environ Microbiol 65:5409–5420

    CAS  Google Scholar 

  • He JZ, Xu ZH, Hughes J (2005) Pre-lysis washing improves DNA extraction from forest soils. Soil Biol Biochem 37:2337–2341

    Article  CAS  Google Scholar 

  • Krsek M, Wellington EMH (1999) Comparison of different methods for the isolation and purification of total community DNA from soil. J Microbiol Meth 39:1–16

    Article  CAS  Google Scholar 

  • Martin-Laurent F, Philippot L, Hallet S, Chaussod R, Germon JC, Soulas G et al (2001) DNA extraction from soils: old bias for new microbial diversity analysis methods. Appl Environ Microbiol 67:2354–2359

    Article  CAS  Google Scholar 

  • Porteous LA, Armstrong JL (1991) Recovery of bulk DNA from soil by a rapid, small-scale extraction method. Curr Microbiol 22:345–348

    Article  CAS  Google Scholar 

  • Smalla K, Cresswell N, Mendoncahagler LC, Wolters A, Vanelsas JD (1993) Rapid DNA extraction protocol from soil for polymerase chain reaction-mediated amplification. J Appl Bacteriol 74:78–85

    CAS  Google Scholar 

  • Torsvik VL (1980) Isolation of bacterial-DNA from soil. Soil Biol Biochem 12:15–21

    Article  CAS  Google Scholar 

  • Torsvik VL, Ovreas L, Thingstad TF (2002) Prokaryotic diversity—magnitude, dynamics, and controlling factors. Science 296:1064–1066

    Article  CAS  Google Scholar 

  • Tsai YL, Olson BH (1991) Rapid method for direct extraction of DNA from soil and sediments. Appl Environ Microbiol 57:1070–1074

    CAS  Google Scholar 

  • Whitman WB, Coleman DC, Wiebe WJ (1998) Prokaryotes: the unseen majority. Proc Natl Acad Sci USA 95:6578–6583

    Article  CAS  Google Scholar 

  • Zhang L, Xu ZH (2008) Assessing bacterial diversity in soil: a brief review. J Soils Sediments 8:379–388

    Article  CAS  Google Scholar 

  • Zhou JZ, Bruns MA, Tiedje JM (1996) DNA recovery from soils of diverse composition. Appl Environ Microbiol 62:316–322

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. INRA, Université de Bourgogne, Soil and Environmental Microbiology, 21065, Dijon cedex, France

    Laurent Philippot, Philippe Lemanceau & Fabrice Martin-Laurent

  2. DACPA, Sezione di Scienze Agrochimiche, University of Catania, Via Santa Sofia 98, 95123, Catania, Italy

    Cristina Abbate

  3. ADEME, Waste and Soil Research Dpt, 49004, Angers Cedex 01, France

    Antonio Bispo

  4. Laboratoire Etudes et Expertises, IPL santé, environnement durables Est, 54521, Maxéville, France

    Thierry Chesnot

  5. Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, 750 07, Uppsala, Sweden

    Sara Hallin

  6. Department of Food and Environmental Sciences, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland

    Kristina Lindström

  7. INERIS, Parc Technologique ALATA, BP 2, 60550, Verneuil en Halatte, France

    Pascal Pandard

  8. CSIC, Estacion Experimental del Zaidín, Environmental Protection, 18008, Granada, Spain

    Esperanza Romero

  9. Department for Terrestrial Ecogenetics Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), 85764, Neuherberg, Germany

    Michael Schloter

  10. Environmental Microbial Genomics Group, Laboratoire AMPERE, Ecole Centrale de Lyon, Université de Lyon, 69134, Ecully, France

    Pascal Simonet

  11. Julius Kühn-Institut Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, 38104, Braunschweig, Germany

    Kornelia Smalla

  12. Department of Ecology, Technical University Berlin, 10587, Berlin, Germany

    Berndt-Michael Wilke

  13. Wellience Agro-Environment, BP 66517, 21065, Dijon Cedex, France

    Imes Petric & Fabrice Martin-Laurent

Authors
  1. Laurent Philippot
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  2. Cristina Abbate
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  3. Antonio Bispo
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  4. Thierry Chesnot
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  5. Sara Hallin
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  6. Philippe Lemanceau
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  7. Kristina Lindström
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  8. Pascal Pandard
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  9. Esperanza Romero
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  10. Michael Schloter
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  11. Pascal Simonet
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  12. Kornelia Smalla
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  13. Berndt-Michael Wilke
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  14. Imes Petric
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  15. Fabrice Martin-Laurent
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Corresponding author

Correspondence to Fabrice Martin-Laurent.

Additional information

Responsible editor: Jean Louis Morel

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Philippot, L., Abbate, C., Bispo, A. et al. Soil microbial diversity: an ISO standard for soil DNA extraction. J Soils Sediments 10, 1344–1345 (2010). https://doi.org/10.1007/s11368-010-0265-8

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  • DOI: https://doi.org/10.1007/s11368-010-0265-8

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