Abstract
Coxsackievirus B3 (CVB3) is a common factor in human myocarditis. The interplay between host factors and virus components is crucial for the fate of the infected cells. Despite that, host protein responses, which characterize CVB3-induced diseases, have not yet been determined in detail. To investigate the nature of modified protein patterns in infected human cells compared with uninfected cells, two-dimensional gel electrophoresis in combination with matrix-assisted laser desorption/ionization-mass spectrometry were used. The regulated proteins, e.g. nucleophosmin (nucleolar protein B23), lamin, the RNA-binding protein UNR and the p38 mitogen-activated protein kinase, were sorted according to their functional groups and interpreted in the context of the myocarditis process.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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Coxsackievirus Infections / etiology
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Coxsackievirus Infections / metabolism*
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DNA-Binding Proteins / isolation & purification
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DNA-Binding Proteins / metabolism
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Electrophoresis, Gel, Two-Dimensional
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Enterovirus B, Human / classification
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Enterovirus B, Human / pathogenicity*
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HeLa Cells
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Humans
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Lamins / isolation & purification
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Lamins / metabolism
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Myocarditis / etiology
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Myocarditis / metabolism
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Nuclear Proteins / isolation & purification
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Nuclear Proteins / metabolism
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Nucleophosmin
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Proteome / isolation & purification
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Proteome / metabolism*
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RNA-Binding Proteins / isolation & purification
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RNA-Binding Proteins / metabolism
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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p38 Mitogen-Activated Protein Kinases / isolation & purification
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p38 Mitogen-Activated Protein Kinases / metabolism
Substances
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CSDE1 protein, human
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DNA-Binding Proteins
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Lamins
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NPM1 protein, human
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Nuclear Proteins
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Proteome
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RNA-Binding Proteins
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Nucleophosmin
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p38 Mitogen-Activated Protein Kinases