Post-translational His-Cys cross-linkage formation in tyrosinase induced by copper(II)-peroxo species

Nobutaka Fujieda; Takuya Ikeda; Michiaki Murata; Sachiko Yanagisawa; Shigetoshi Aono; Kei Ohkubo; Satoshi Nagao; Takashi Ogura; Shun Hirota; Shunichi Fukuzumi; Yukihiro Nakamura; Yoji Hata; Shinobu Itoh

doi:10.1021/ja108280w

Post-translational His-Cys cross-linkage formation in tyrosinase induced by copper(II)-peroxo species

J Am Chem Soc. 2011 Feb 9;133(5):1180-3. doi: 10.1021/ja108280w. Epub 2011 Jan 10.

Authors

Nobutaka Fujieda¹, Takuya Ikeda, Michiaki Murata, Sachiko Yanagisawa, Shigetoshi Aono, Kei Ohkubo, Satoshi Nagao, Takashi Ogura, Shun Hirota, Shunichi Fukuzumi, Yukihiro Nakamura, Yoji Hata, Shinobu Itoh

Affiliation

¹ Department of Material and Life Science, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.

PMID: 21218798
DOI: 10.1021/ja108280w

Abstract

Autocatalytic formation of His-Cys cross-linkage in the enzyme active site of tyrosinase from Aspergillus oryzae has been demonstrated to proceed by the treatment of apoenzyme with Cu(II) under aerobic conditions, where a (μ-η(2):η(2)-peroxo)dicopper(II) species has been suggested to be involved as a key reactive intermediate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aspergillus oryzae / enzymology
Biocatalysis
Copper / chemistry*
Cross-Linking Reagents / chemistry
Cross-Linking Reagents / pharmacology
Cysteine / chemistry*
Histidine / chemistry*
Models, Molecular
Monophenol Monooxygenase / chemistry*
Monophenol Monooxygenase / metabolism*
Organometallic Compounds / chemistry
Organometallic Compounds / pharmacology*
Protein Conformation
Protein Processing, Post-Translational / drug effects*

Substances

Cross-Linking Reagents
Organometallic Compounds
Histidine
Copper
Monophenol Monooxygenase
Cysteine