Abstract
Leaf lesions of Mandevilla sanderi were shown to be caused by Pseudomonas savastanoi. While BOX fingerprints were similar for P. savastanoi isolates from different host plants, plasmid restriction patterns and sequencing of plasmid-located pathogenicity determinants revealed that Mandevilla isolates contained similar plasmids distinct from those of other isolates. A repA-based detection method was established.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apocynaceae / microbiology*
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Bacteriological Techniques / methods
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DNA Helicases / genetics
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Molecular Sequence Data
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Plant Diseases / microbiology*
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Plant Leaves / microbiology
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Plasmids / analysis
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Polymorphism, Restriction Fragment Length
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Pseudomonas / isolation & purification*
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Pseudomonas / pathogenicity*
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Sequence Analysis, DNA
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Trans-Activators / genetics
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Virulence Factors / genetics
Substances
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DNA, Bacterial
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Trans-Activators
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Virulence Factors
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replication initiator protein
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DNA Helicases
Associated data
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GENBANK/JX227983
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GENBANK/JX227984
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GENBANK/JX227985
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GENBANK/JX227986
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GENBANK/JX678983