Quantification of IncP-1 plasmid prevalence in environmental samples

Sven Jechalke; Simone Dealtry; Kornelia Smalla; Holger Heuer

doi:10.1128/AEM.03728-12

Quantification of IncP-1 plasmid prevalence in environmental samples

Appl Environ Microbiol. 2013 Feb;79(4):1410-3. doi: 10.1128/AEM.03728-12. Epub 2012 Dec 14.

Authors

Sven Jechalke¹, Simone Dealtry, Kornelia Smalla, Holger Heuer

Affiliation

¹ Julius Kühn-Institut-Federal Research Center for Cultivated Plants, Department of Epidemiology and Pathogen Diagnostics, Braunschweig, Germany.

Abstract

To study the role of broad-host-range IncP-1 plasmids in bacterial adaptability to irregular environmental challenges, a quantitative real-time PCR assay was developed that specifically detects the korB gene, which is conserved in all IncP-1 plasmids, in environmental samples. IncP-1 plasmid dynamics in a biopurification system for pesticide wastes were analyzed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics
Bacteroidetes / genetics*
Biotransformation
Environmental Microbiology*
Pesticides / metabolism
Plasmids / analysis*
Plasmids / classification
Real-Time Polymerase Chain Reaction

Substances

Bacterial Proteins
KorB protein, Plasmid RK2
Pesticides