First Report of blaIMP-14 on a Plasmid Harboring Multiple Drug Resistance Genes in Escherichia coli Sequence Type 131

Nicole Stoesser; Anna E Sheppard; Gisele Peirano; Robert P Sebra; Tarah Lynch; Luke W Anson; Andrew Kasarskis; Mary R Motyl; Derrick W Crook; Johann D Pitout

doi:10.1128/AAC.00840-16

First Report of blaIMP-14 on a Plasmid Harboring Multiple Drug Resistance Genes in Escherichia coli Sequence Type 131

Antimicrob Agents Chemother. 2016 Jul 22;60(8):5068-71. doi: 10.1128/AAC.00840-16. Print 2016 Aug.

Authors

Affiliations

¹ Modernizing Medical Microbiology Consortium, Nuffield Department of Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom nicole.stoesser@ndm.ox.ac.uk.
² Modernizing Medical Microbiology Consortium, Nuffield Department of Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom.
³ Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada.
⁴ Icahn Institute and Department of Genetics and Genomic Sciences, Icahn School of Medicine, Mount Sinai, New York, New York, USA.
⁵ Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada.
⁶ Clinical Microbiology, Merck and Co. Inc., Rahway, New Jersey, USA.
⁷ Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada Snyder Institute for Chronic Diseases, University of Calgary, Calgary, Alberta, Canada Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa.

Abstract

The blaIMP-14 carbapenem resistance gene has largely previously been observed in Pseudomonas aeruginosa and Acinetobacter spp. As part of global surveillance and sequencing of carbapenem-resistant Escherichia coli, we identified a sequence type 131 strain harboring blaIMP-14 within a class 1 integron, itself nested within an ∼54-kb multidrug resistance region on an epidemic IncA/C2 plasmid. The emergence of blaIMP-14 in this context in the ST131 lineage is of potential clinical concern.

MeSH terms

Acinetobacter / drug effects
Acinetobacter / enzymology
Acinetobacter / genetics
Anti-Bacterial Agents / pharmacology
Carbapenems / pharmacology
Drug Resistance, Multiple, Bacterial / genetics
Escherichia coli / drug effects*
Escherichia coli / enzymology*
Escherichia coli / genetics
Integrons / genetics
Microbial Sensitivity Tests
Plasmids / genetics*
Pseudomonas aeruginosa / drug effects
Pseudomonas aeruginosa / enzymology
Pseudomonas aeruginosa / genetics
beta-Lactamases / genetics
beta-Lactamases / metabolism*

Substances

Anti-Bacterial Agents
Carbapenems
beta-Lactamases

Abstract

MeSH terms

Substances

Grants and funding