Protein Enrichment from Highly Dilute Samples with StrataClean

Florian Bonn; Andreas Otto

doi:10.1007/978-1-4939-8695-8_2

Protein Enrichment from Highly Dilute Samples with StrataClean

Methods Mol Biol. 2018:1841:11-18. doi: 10.1007/978-1-4939-8695-8_2.

Authors

Florian Bonn¹, Andreas Otto²

Affiliations

¹ Institute of Biochemistry II, University Hospital, Goethe University School of Medicine, Frankfurt, Germany. bonn@med.uni-frankfurt.de.
² Department of Microbial Proteomics, Institute for Microbiology, University Greifswald, Greifswald, Germany.

PMID: 30259476
DOI: 10.1007/978-1-4939-8695-8_2

Abstract

Efficient and reproducible protein enrichment is a prerequisite for the proteomic analysis of highly dilute solutions like culture supernatant or body fluids. Traditionally several precipitation strategies were used for this purpose, but they have certain drawbacks. Here, a protocol for StrataClean enrichment-a very efficient and easy-to-use method for the enrichment of proteins from highly dilute samples-which is compatible to gel-free proteomics, is presented.

Keywords: Highly dilute samples; Protein enrichment; Solid-phase enrichment; StrataClean.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Proteins / isolation & purification*
Proteomics / methods*
Solid Phase Extraction
Workflow

Substances

Proteins