We report on a 72-year-old patient with a clinically diagnosed plasmocytoma which developed to a plasma cell leukemia (PCL) with so far unrecorded complex translocations. As GTG-banding was not able to resolve all karyotypic changes, multiplex-fluorescence in situ hybridization (M-FISH) in combination with microdissection based comparative genomic hybridization (micro-CGH) and multicolor banding (MCB) have been done. Using these molecular cytogenetic approaches the karyotype of the PCL case can be described as: 51,XY,-1,-1,+3,+der(5)t(5;11;1)(5pter↷5q13-q14::11q24↷11q25::1q12↷1qter),+7 or +der(7)t(7;1)(7qter↷7p15::1p31.1↷1pter),+8,+der(9)t(1;9)(1qter↷1q12::9q12↷9pter),der(11)t(1;11;1)(1pter↷1p31.1::11p15.5↷11q25::1q12↷1qter),-13,der(14)t(X;14)(Xqter↷Xq21.3::14pter↷14qter),+15,+18,der(19)t(9;19)(9qter↷9q12::19q11↷19pter),+i(19)(q10). The case shows one of the most complex karyotypic rearrangements ever described in PCL and indicates two additional chromosomal regions which may contain genes of interest for the development of this hematological disorder: loss of 1p10-p31.1 material and gain of Xq21.3-qter.

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