Plant Methods (Dec 2011)

Reverse protection assay: a tool to analyze transcriptional rates from individual promoters

  • Zubo Yan O,
  • Kusnetsov Victor V,
  • Börner Thomas,
  • Liere Karsten

DOI
https://doi.org/10.1186/1746-4811-7-47
Journal volume & issue
Vol. 7, no. 1
p. 47

Abstract

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Abstract Transcriptional activity of entire genes in chloroplasts is usually assayed by run-on analyses. To determine not only the overall intensity of transcription of a gene, but also the rate of transcription from a particular promoter, we created the Reverse RNase Protection Assay (RePro): in-organello run-on transcription coupled to RNase protection to define distinct transcript ends during transcription. We demonstrate successful application of RePro in plastid promoter analysis and transcript 3' end processing.

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